Screening Of The Protein Related To Nucleoside Antibiotics Biosynthesis In Micromonospora Carbonacea JXNU-1

Micromonospora carbonacea JXNU-1 with broad-spectrum antibacterial activity was isolated by our laboratory. The nucleoside antibiotics produced by M. carbonacea JXNU-1 exhibited the antibacterial activity against some Gram-positive and Gram-negative bacteria. However the biosynthesis and molecular regulation mechanism of the antibiotics were still unclear. In this paper, the biosynthesis and molecular regulation mechanism of antibiotics in M. carbonacea JXNU-1 were explored by the iTRAQ. The results are as follows:(1) A total of 2390 proteins in M. carbonacea JXNU-1 were identified. There were 172 differentially expressed proteins related to the nucleoside antibiotics produced by M. carbonacea JXNU-1 including 76 up-regulated proteins and 96 down-regulated proteins.(2) Based on the analysis of proteomics, twelve proteins related to the synthesis of antibiotics were obtained, such as ABC transporter substrate-binding protein, ABC transporter ATP-binding protein, glycosyltransferase, P450 monooxygenase, SAM-dependent methyltransferase, serine hydroxymethyltransferase and methyltransferase. Real-time quantitative reverse transcription PCR was used to quantitative the mRNA of related proteins. It was showed that the twelve genes were up-regulated, which was consistent with the expression of proteins.(3) The above twelve genes had been mapped by bioinformatics technology. Results showed that the proteins related to the synthesis of antibiotics were distributed in 5 gene clusters, and the cluster 2 in M. carbonacea JXNU-1 could be identified as the gene cluster for antibiotics biosynthesis.The above results provided the theoretical basis for revealing the biosynthesis and molecular regulation mechanism of the antibiotics in M. carbonacea JXNU-1.