Study On The Volatile Analysis Of The Flowers And Leaves In Plants By Gas Chromatography-Mass Spectrometry

There are thousands of species of plants in nature. Different plant organs are endowed with unique volatile components. The people's daily life can be fully benefited through studying the composition characteristics and differences of these components and further achieving reasonable development and application. In this paper, using GC-MS as detecting method, two sample pretreatment techniques of self-made device solvent extraction(SMD-SE) and headspace solid phase microextraction(HS-SPME) as the capturing and enrichment method for the target components, the composition of the volatile components in the following three plant systems was studied.(1) Using Syringa Linn. as the detection sample, the analytical method for the the volatile component composition of its 16 varieties was established. For the sample pretreatment technique, the extraction performance of SMD-SE for the volatile compounds was evaluated through comparing with HS-SPME which is used as the reference standard; for the component separation means, the good separation was achieved for the target component system using the temperature programmed technique of the capillary column based on the difference of the boiling point and the polarity; for the result analysis method, using the auto mass deconvolution & identification system(AMDIS) as the aided method, the identification method of the mass spectrum matching degeree integrating with the the retention index was used, which can gurantee the accuracy for identifing the target components; meanwhile, the relative content of the different components in the target system was confirmed using the peak area normalization method.(2) Using the Tulip as the detection sample, the analytical method for the the volatile component composition of its 7 varieties was established. The key parameters which can affect the extraction efficiency of the volatile components in the sample were optimized, and the extraction performance of the target components for two methods, SMD-SE and HS-SPME, was compared and analyzed; the separation method for the target components using the capillary column temperature programmed technique was established; the identification method using the mass spectrum matching degree integrating with the retention index was constructed and the relative content quantitative method for the target components using the peak area normalization means was established. Under the optimized SMD-SE extraction conditions, 11 kinds of compounds were separated and identified from 7 target systems, thereby confirming the "skeleton" of the volatile constituents for the Tulipa.(3) Using the ageratum as the detection sample, the analytical method for the the volatile component composition of its flower and leaf was separately established. For the sample pretreatment method, the extraction performance of the system volatile compounds for two methods, SMD-SE and HS-SPME, was compared. The research results showed that under the optimized conditions, SMD-SE shows a better performance than HS-SPME for the flower system and is slightly inferior to HSSPME for the leaf system, which shows that SMD-SE has a good potential for the extraction of specific volatile components.