Heterologous Expression And Bioinformatics Analysis Of Biosynthetic Gene Cluster Of Xiamenmycin In Streptomyces Hygroscopicus FR008 And Streptomyces Albus

One benzopyran compound(xiamenmycin) was obtained from Streptomyces xiamenensis 318 sourced from mangrove sources by our group. And we proved that xiamenmycin can inhibit scar formation and fibroblast proliferation and suppresses inflammatory cell retention. So xiamenmycin can be a potential medicine in anti-fibrosis and anti-inflammatory.Owing to Streptomyces xiamenensis 318 produces a small quantity of xiamenmycin, we need to find adaptive hosts for heterologous expression of xiamenmycin. Streptomyces hygroscopicus FR008 is fermented in short time, and has been proved to be applicable to the potential for industrial production, and its genetic background is clear compared with Streptomyces xiamenensis 318. Streptomyces albus has been used to study in industrial production for a variety of pigments and antibiotics. We introduce the xiamenmycin biosynthesis gene cluster into Streptomyces albus and Streptomyces hygroscopicus FR-008. And the xiamenensis is obtained in the Streptomyces albus and Streptomyces hygroscopicus FR008Because there is no investigation on optimization of medium for Streptomyces xiamenensis 318. We screen the medium for Streptomyces xiamenensis 318. And owing to the yield of xiamenmycin of Streptomyces albus-09404 is little, we just preliminary study the optimization of medium for Streptomyces hygroscopicus FR008-09404. The yield of xiamenmycin of Streptomyces xiamenensis 318 in SFM medium is highest. And The yield of xiamenmycin of Streptomyces hygroscopicus FR008-09404 in No.1 medium is 2.5-fold than that of Streptomyces xiamenensis 318 in SFM medium. We find that soybean powder, yeast extract, malt extract, glucose, NaCl?K2HPO4?FeSO4·7H2O and MgSO4·7H2O are significant factors, and glycerol, L-Asp and L-Tyr are little effect on xiamenmycin.The structures of proteins in the xiamenmycin biosynthesis gene cluster are not clear. Therefore this article take these proteins as the research objects, discussed the potential structures and the functions by means of bioinformatics. We analyze their potential structures and functions, including transmembrance domains, secondary structures, tertiary structures, subceller localizations, protein fingerprints. This article establishes theoretical basis for further research of pathogenesis of biosynthesis of xiamenmycin.