Light Regulation Of FOF2 Gene And Their Preliminary Functional Analysis In Arabidopsis Thaliana

Many members of the family of F- box protein in plants, the F- box structure domain as the substrate protein specificity recognition site, mediated the substrate protein degradation by ubiquitin degradation pathways and regulate the important life activities of plant, including plant signal transduction of molecules, the formation of plant organs and stress response of plant. External environment affects plant growth and development, then the light as a major environmental element, Light is not only a necessary sources of energy in green plant photosynthesis and can be used as a signal molecular in regulation of plant growth and development process, in order to plant better adapt to the environment. The evolution of higher plants have a fine feeling of light and signal system, to monitor the direction of light signal, quantity and quality to adjust their growth and development. A large number of studies have shown that blue light receptor CRY and red/far-red light receptor PHY mediated blue and red/far-red light to regulate plant growth and development, including the inhibition of hypocotyl elongation, cotyledon opening, stomatal opening, anthocyanin and the regulation of gene expression. This research through the FOF2 gene expression patterns and the preliminary research of the function, explore FOF2 genes in plant growth and development of the optical signal pathway and function. Then to obtain the following results:Analyze the effect of light on FOF2 gene expression found that FOF2 gene expression induced by blue light, and the expression amount increasing with the increase of blue light time; Also found that under red light treatment, FOF2 expression of transient increase, showed the red light to the gene expression of the instantaneous entrainment; And under far red or dark treatment, the gene transcription level drops, protein levels is degraded, far-red light inhibit FOF2 expression, the expression has the light quality specificity. It was found that under the dark, FOF2 protein degradation associated with proteasome pathway.Analysis FOF2 genetic influence on the growth and development in arabidopsis, found under the red and white light processing, missing the hypocotyl of mutant plants significantly shorter than the wild type, and dark, blue and far red down the length of hypocotyl no difference, there may be a specific reaction showed that the gene for red light.Through quantitative PCR analysis of FOF2 gene in tissue specific expression model found that it expreesed in every organs, but express higher in roots?cauline leaf and sililques,speculation FOF2 may participate in regulating the growth process of arabidopsis thaliana leaves and sililques.By comparing the growth situation of FOF2 overexpression lines?fof2 mutant and wild type plant, found that overexpression FOF2 caused the increase of leaf size, then the mutant plant leaf was no any significant change than wild type. Also found that the chlorophyll content of the transgenic plants is no difference between wild type. FOF2 overexpression transgenic rosette leaves number is more than the wild type and the flowering time than the wild type delay; then the rosette leaves number and flowering time of FOF2 lack mutant plants compare with wild type was no too big change;Than detection of FOF2 gene expression in different development periods, found that as for the vegetative growth stage gene expression fall after rise first;Detection the promoting flowering factor of AP1 and FT expression in FOF2 overexpression plants found that its expression was inhibited. Suggest that FOF2 gene can regulate vegetative growth period in arabidopsis thaliana, promote blade increases, inhibiting bloom at the same time, is the negative regulation factor in flowering pathway, then its may be related to the AP1 or FT pathway.