Preliminary Application Of Toehold Mediated Strand Displacement And Silver Nonoclusters In Biology And Environment Detection

Along with the social progress and improvement of living level, people are increasingly demanding for health. As we all know, biological macromolecules involved in many important biological processes, Some changes of biological macromolecules could herald a disease or initiate irreversible changes in some physiological process. At the same time, some environmental factors can also influence people's health, especially the heavy metal in environment bear great harm for people, even though it exists in the form of the trace. Therefore, The detection of biological molecules and heavy metal of environment has very important significance in disease diagnosis, environmental safety and other areas of the people's livelihood. At present, own with easy operation and moderate cost of Toehold-mediated strand displacement, it received great attention in the biological computers and biological molecular detection. Besides, the new development technology of silver nanoclusters are becoming more and more important in sample analysis because of its high quantum yield and good light stability. Based on this, this paper develop new detection system of biological macromolecules and heavy metal using Toehold-mediated strand displacement and silver nanoclusters technology for a preliminary application research. The detailed content as follows:1 ?We establish a fluorescence detection method for the point mutation based on toehold-mediated strand displacement and microspheres capture. Many hereditary disease is closely related to point mutations, the accurate detection of point mutations has important significance for the prevention of diseases, clinical detection and molecular diagnosis. Currently, Toehold-mediated strand displacement based on fluorescence polarization can quickly realize the point mutation detection, but it need expensive fluorescence detection system. We design a new detection method for realizing fast detection of point mutation based on Toehold-mediated strand displacement and microspheres capture. This method can apply in much types of point mutation detection and not need to expensive instruments as well as electrophoresis, centrifugation, Separation, enzyme supplementation and so on. It is expected to play an important role in clinical detection and application.2?A method for the detection of Exonuclease I based on G-rich amplify fluorescence signal of silver nanoclusters has been develop. Exonuc lease I can degrade nucleotides from one end of nucleotide strand, it has important significance in analysising nucleic acid structural differences, determinating special position of genome DNA and participating in some important biological process. Traditional detection method of enzyme consist of gel electrophoresis, colorimetric method, radioactive labeling method and enzyme-linked immunosorbent method, etc. It usually require complicated operation process or expensive equipment.Based on G-rich can greatly amplify fluorescence signal of silver nanoclusters,we develop a kind of new detection system to realize the rapid and sensitive detection of Exonuclease ?. The sensitivity of this method is 0.05 U/mL with a better specificity. Besides, it does not need to suffer complex operation and expensive instruments. Moreover, it provides a good detection strategy for the analysis and research of the protein enzyme, at the same provides an effective method for the enzyme activity of the inhibitor screening.3?A method for the detection of mercury ions based on Toehold-mediated strand displacement and silver nanoclusters technology has been develop. Mercury ions have serious harm to human health and the environment so it is important to detect the content of mercury ion in the environment for human ecological management, sewage dischargement, industrial and domestic water, etc. Traditional detection method of mercury ions are mainly based on spectrum or mass spectrometry, it suffer large and expensive instruments and high requirement for purity, preparation of the sample, besides it is difficult to meet the demand for real-time detection. We establish a new detection method of mercury ions based on Toehold-mediated strand displacement and silver nanoclusters technology. This method take advantage of simple operation, low cost. At the same time, it can realize the actual detection of mercury ion in water samples and provide a good strategy for real-time monitoring of heavy metal ions in the environment.