| Modern radical research showed that the organism aging, cancer, inflammation and autoimmune diseases are related with free radical metabolism imbalance, marine microbial polysaccharide has good antioxidant activity, it is an effective way to to find the natural antioxidant drugs.Three yeast strains of exopolysaccharide-producing, named HS-J6, HS-J8 and HS-J9, were isolated from sea cucumber intestine.Three water-soluble polysaccharides, named EPS6, EPS8 and EPS9, were extracted by95% ethanol precipitation, deproteinization via sevage, dialysis and lyophilization. The analysis of the structural characteristics for EPS6, EPS8 and EPS9 was studied by infrared spectral technology. It showed that there was obvious characteristic absorption peaks of polysaccharides. The antioxidation of extracellular polysaccharide was slso done in this study.The results showed that the reduction capacity of EPS6 and EPS8 were significantly higher than that of EPS9. But EPS9 exhibited high scavenging effects on O2-·radicals, with the maximum scavenging rates about 35.34%. The ·OH radical scavenging activity of EPS6 was the highest, with the scavenging rate about 91.46%.Sea cucumber was a main aquateic breeding species in the north of our country. It is emergent to find new feed additives with high efficiency, safety and no residue to increase the rate of growth.Compared with the control group feed with only bait, EPS9 was choosen as experimental group to feed sea cucumber as an additive. The results showed that the experimental group was better than the control group in a series of indicators,such as body weight and enzyme activityin intestinal digestive. This study laid the foundation for the further research and application for extracellular polysaccharide from marine yeasts. |
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