| DNA barcodes and morphometrics of some species of the genus Pachycondyla were studied.The main results were as follows:1.The morphological characteristics of 13 species of the genus Pachycondyla were analyzed based on morphometric measurement.The results showed that 13 species could be clustered into four clades,the first clade included 4 species(P.brevidorsa,P.melanari,P.rufipes,P.leeuwenhoeki),and having the highest similarity;the second clade comprised of 3 species(P.lobocarena,P.zhengi,P.astuta),followed by the third clade,which with lower similarity,formed by 4 species(P.pilosior,P.annamita,P.sauteri,P.javana);and finally the lowest similarity,the fourth clade.P.chinensis and P.luteipes have the closest relationship,clustered into a clade;P.zhengi and P.astuta;P.sauteri and P.javana have close relationships and clustered into another clade.2.The barcode sequences of CO1,ITS1 and their combined datasets were analyzed using Neighbor-Joining(NJ),Maximum Parsymany(MP)and Bayesian Inferences(BI)methods.The results showed that all groups with enough samples formed monophyletic clades in the phylogenetic trees at the species level(the species with significant differentiations of morphological characters).Furthermore,their phylogenetic relationships:(P.chinensis + P.luteipes)+(P,astuta + P.zhengi + P.javana + P.sauteri + P.lobocarena)+(P.leeuwenhoeki + P.pilosior + P.melanaria + P.rufipes)by the similar topology structure.3.The comparison analyses of some species of the genus Pachycondyla based on DNA barcoding and morphometrics.The results showed that phylogenetic analyses were consistent with morphological studies.P.chinensis and P.luteipes having the highest similarity from each other;also,P.astuta,P.zhengi,P.javana,P.sauteri and P.lobocarena have close relationships from one to another(except some species with litter differentiations of morphological characters).Further study is needed to confirm their phylogenetic relationships. |
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