| With the development of science and technology, more and more concerns are focused on human life sciences, and people are also paying attention to any tiny thing that related authigenic health. The blood cell analyzer is the base in health products. It is an important tool in the diagnosis of health. Through the cell classification,recognition and counting, find the pathogenesis ultimately. In the 21 st century, the health industry has been listed as the strategic industry in many countries and it is not only challenges but also opportunities for the development of China.Blood cell analyzer is the typical product in the health industry. Mostly, laser scattering technology and chemical dyeing technology are applied to today’s blood cell analyzers. The chemical staining has many shortcomings: larger cells dosage which is not suitable for the detection of precious cells; it’s volume is so big to carrying, so that an emergency can’t exert it’s maximum effect; in addition, the fluorochrome is used in the fluorescent dye, which has certain effects on the activity of cells. But laser scattering technique, with the advantages of quantitative,non-invasive and no damage, provides a very powerful tool for the quantitative study of biological cells. The intensity of light scattering signals from all directions, which are received by photoelectric detectors that based on Mie scattering theory, are input into computer for analysis.People developed the technology of microfluidic chips in existing cell analysis technology, and it makes the blood cell analysis into the new field of research.Microfluidic chip is a kind of science and technology that can control the fluid in the micron scale space, and it has the ability to integrate the basic function of biological,chemical and other laboratories on the chip of just a few square centimeters, so we also call it “Lab on a chip”. The volume of equipment will greatly decreased if the microfluidic chip technology is melted into the blood cell analyzer, and it will make the health product easy to carry, which can make people’s life more convenient and quality improved.The target of this paper is mainly focused on the high precision accuracy andminiaturization of the hematology analyzer. We used numerical simulation technology to get the forward scattering intensity signal distributions of spherical models with different size, including diameters of 10μm, 14μm and 20μm. Thus, volume identification and classification can be made based on the screen results. We designed a blood cell analysis sensor based on the target of miniaturization, resource recovery and accuracy. This sensor fully utilized the backscatter light of the cells, increased the recognition of basis, and also increased the intensity of scattering signal through measuring the scattering intensity of the solid angle range cell signal, which improved the detection accuracy. And we optimized the design of the traditional microfluidic chip structure through the Solidworks modeling software, made different structures of the sag up and down the channel walls, including flow plate type,ellipsoid and cylindrical. The fork type optical flow control structure combined with sheath flow technology ensured the cell pass the laser detection area one by one.The optimal design of cylindrical sag was finally determined through using Ansys to simulate internal fluid flow of the optical flow control chip by comparing the diagrams of phase, velocity and tracing particles. The experiment results show that this design avoided the detection error caused by cell position overlap, avoided the internal suddenly raised and the internal fluid turbulence effect in the channels due to the buffer structure, and ultimately improved the accuracy of the test results, achieved the goal of miniaturization and improved accuracy.As a result of the limitation of my ability, blood cell analyzer research road is very long, and there are still many problems should be solved, this will inspire us to further research in the future. |
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