Design, Construction Of Self-powered Biosensor And Their Application In Biological Analysis

Paper-based biosensors are a new alternative technology for fabricating simple, low-cost, portable and disposable analytical devices for many application areas including clinical diagnosis, environmental monitoring and food quality control. However, most of the paper-based biosensors relied on the external power sources to generated detection signal, which restricted the wide extension and point-of-care testing. Thus, there is a great desire to development on-site diagnostics without external power sources, simplifying the fabrication process, minimizing the scale, and reducing the expense, which is especially beneficial to the miniaturization of detection devices.In this paper, to realize the point of care testing, rapid and portable determination, we combined the photoelectrochemistry and biofuel cell with microfluidic paper-based analytical devices to development simple, low-cost and portable device. The main research is as follows:(1) CdTe quantum dots dotted molecular imprinted polymers was synthesized and immobilized on the gold nanoparticles modified paper working electrode. Molecular imprinting-photoelectrochemistry was introduced into microfluidic paper-based analytical device to design a novel paper-based photoelectrochemical(paper-based PEC) protocol to implement pesticide detection.(2) A novel microfluidic photoelectrochemical(PEC) paper-based analytical platform, integrated with a fluidic delay-switch, an internal chemiluminescent light source, a novel paper supercapacitor amplifier, and a terminal digital multi-meter detector, for sensitive DNA detection using a graphene-modified porous Au-paper electrode as the working electrode to obtain enhanced PEC responses.(3) A three-dimensional microfluidic origami glucose–air immuno-biofuel cell has been successfully demonstrated for the first time to implement self-powered, sensitive, and low-cost sandwich immunoassay for cancer markers based on the signal amplifications of a porous Au-paper electrode and Au nanoparticles attached to carbon nanotubes.(4) A glucose-air enzymatic biofuel cell was introduced into microfluidic paper-based analytical devices with gold nanoparticles and platinum nanoparticles modified paper electrode as the anodic and cathodic substrate, respectively, to implement self-powered, sensitive, low-cost and simple DNA detection. As a further development of the analytical equipment, an paper supercapacitor was designed and integrated into the BFCfor current amplification, and a terminal digital multi-meter detector was introduced for the terminal current detection.