| Optical projection tomography(OPT) is a new technique for biological imaging of samples which is 0.5 to 10 mm. It is particularly useful for reconstructing vertebrate embryos and examining the 3D anatomy of developing organs. With high spatial resolution, high scanning speed and cost less. Due to the limitation of the Optical system, the depth of field of OPT is too small. It can’t meet the need of biological imaging, so we combined it with wavefront coding to raise the depth of field.Improving the DOF of OPT in biological imaging will make it more extensive application prospect.In this paper we analyzed and compared OPT with other imaging techniques, it proved that the OPT is suit for embryonic, tissues as well as the small scale of biological sample, further analysis the imaging principle and steps of OPT, and talked about the OPT limit of depth of field of the optics system.Discussed the techniques to stretch the depth of field of the Optical systems, in order to get larger depth of field, we choose wavefront coding to combining with OPT. Build large depth of field OPT imaging system platform, analyzed and designed the function of the module of large depth of field OPT, and then studies the key modules about the OPT imaging depths, and according to the actual parameter, based on wavefront coding theory, analyzed the optical module, calculated and designed the surface parameters of spatial light modulator phase modulation under different stretch rates of depth of field. Analyzed large depth of field of OPT is feasibility.Finally we build a large depth of field OPT platform to imaging the biological samples respectively in two times the depth of field stretch rate, five times the depth of field stretch rate, ten times the depth of field stretch rate and the original depth of field. After the image restoration, compared the images between the different depth stretch rates, analyzed the effectiveness of large depth of field OPT. Prove the feasibility of the large DOF OPT system. |
PDF Full Text Request