Quantifying Biochemistry Assays In Paper-based On Micro-Fluidic Device

This thesis describes two different methods for the fabrication of paper-based micro-fluidic devices, onwhich the concentrations of biochemical parameters in solution are detected by combing absorptiometryand image colorimetric methods. The fabrication of the first paper-based micro-fluidic devices includedfollowing two steps: Make the copper mould based on micro-fluidic pattern, which was designed by Protelsoftware. Then, polydimethylsiloxane (PDMS) was coated on the copper mould and solidified in over afterthe PDMS had been permeated in a paper substrate. The fabrication process of anther paper-basedmicro-fluidic devices is as follows: considering the size of reaction zone and without anycross-contamination, the patterns were designed using Protel and transferred to PCB board by hollowingout the PCB board. Wax was filled in the hollowed area in the PCB board, and melted by heating the PCBboard which had been sticked on the mould. The wax could be smooth, even and flat after cool again. Then,a filter paper was placed on the mould and pressed using a glass substrate. At last, the mould, together withthe paper substrate was heated. The melted wax was permeated into the paper substrate and a paper basedmicrofluidicdevice was build.We have also designed an electric circuit for real-time detection of biochemical parameters in solution.The concentrations of the protein and glucose in water solution were demonstrated using the paper-basedmicrofluidic device. The results show that the two methods can generate a hydrophobic zone in themicrofluidic devices. The successful experiments for detection of protein and glucose show that thefabrication methods can be applied to parallel detecting without any cross-contamination when thehydrophilic zone has been filled with identifier. Compared to the standard solution, the errors of the methodare less than2%. The presented methods for the fabrication of micro-fluidic devices and their testingapproaches are simple, inexpensive and less reagents with good linearity. The work is valuable foracademic research, and can be potentially applied in commerce.