A New Auto-microimaging System For Biomedical Analysis

Because electronic technology, control technology and computer technology aredeveloping quickly, optical instruments for automation, intelligent are increasinglydemanding. Drug screening experiments are often carried out in manually withmicroscope. However, this method is very time-consuming, and difficult to achievethe goal of large-scale screening. Therefore, the demand for the development of auto-focus microscope is increasingly urgent. Autofocus microscope has been achieved inthe laboratory. It already has a medical diagnosis, pathological analysis and realteaching function. But it has a small travel and low degree of intelligence, it isdifficult to meet the need of large-scale drug screening.This paper did research and development of biological microscopic imaginganalysis system paper to enable the system to adapt to the rapid drug analysis porousplate automatic intelligent detection based on an optical detection laboratory issue,combined with previous experience in the design of autofocus microscope. Thuscomplete task requirements.The article analyzed the topic request, completed the construction of the medicalautofocus microscopic imaging analysis instrument. And it also designed a largetravel and two-axis scanning platform which can put four pieces of96-well plates. Atthe same time, comparing the characteristics of the traditional mechanicaltransmission, the paper has designed servo motor and voice coil motor (linear motor)mechanical structure of the two levels of regulation, and improved the mechanicalprecision of the microscopic imaging instrument.We studied the AF algorithm or function, tested the performance of threefunctions, variance functions, Laplace function and the maximum gradient valuefunction. Eventually we choose variance functions and Laplace function two kinds offunctions used in parallel considering focusing accuracy and rapidity. Taking intoaccount the focus area for the entire time focusing effects, we designed experimentsto find the optimal focus window.We complete the autofocus based biomedical microscopic imaging analyzersoftware design, enabling the system to complete each hole fast focus and captureimages. Software design is divided into three modules: motion control module,focusing evaluation module and image stitching and storage modules. Motion controlmodule using PC control, signaled by the host computer via multi-axis motion controlcard control motor rotation to complete the motion of the system. Focus evaluationmodule is based on the vc environment to achieve, through the coordination andmotion control modules complete focus tasks. Taking into account the actual needs, specially designed image stitching module, has completed the task of increasing thefield of view. The software is verified by experiment well and the system play well.So the new auto-microimaging system for biomedical analysis is completed.