Effect Of Swimming Training On ACE₂ And AT₁R Of Subfornical Organ In Spontaneous Hypertension Rats

1. PurposeObjective: to find out or improve the long-term moderate-intensity exercise keep hypertensive rats blood pressure with age increase of neural mechanisms.Meaning: to perfect sports theory construction and further human trials of antihypertensive do scientific support.2. MethodIn SHR and WKY as experimental object, the random divided into WKY group(WKYsed), WKY group(WKYext), SHR control group(SHRsed) and SHR group(SHRext), the experiment to WKYext and SHRext swimming intervention for 8weeks.And using noninvasive caudal artery blood pressure tester tests each swimming before intervention, swimming intervention in 4 weeks and 8 weeks after the beginning of the systolic and diastolic blood pressure.By HPLC(High Performance Liquid Chromatography, HPLC) test groups in swimming after the intervention in the urine of norepinephrine levels;Using enzyme-linked Immunosorbent Assay(Enzyme linked Immunosorbent Assay, ELISA) tests between groups in swimming after the intervention of the plasma angiotensin Ⅱ concentration;With Immunofluorescence(IF)Immunofluorescence, test groups in swimming after the intervention under the dome of ACE2 and AT1 R protein content;With immune protein imprinting method(Western blotting, WB) detection in swimming after the intervention of SFO ACE2, AT1 R and gp91 phox protein content.3. Results3.1 blood pressure of all group’sAfter 4 weeks exercise intervention SHRext systolic and diastolic blood pressure significantly below SHRsed, and there were more significant difference(P < 0.05);After the exercise intervention 8 weeks SHRext systolic and diastolic blood pressure significantly below SHRsed, and there were more significant difference(P < 0.05).3.2 after exercise intervention in plasma Ang Ⅱ and content of NE in urineAfter eight weeks of swimming after the intervention in SHRext plasma Ang Ⅱand urine significantly lower content of NE in SHRsed, and there is significant difference(P < 0.05), and particularly significant difference(P < 0.01).3.3 IF the test between groups of ACE2 and AT1 R content in SFO.After eight weeks of swimming after the intervention of SFO SHRext fluorescent protein expression of ACE2 content significantly higher than that of SHRsed, and thereis more significant difference(P < 0.05);Amount of SFO in fluorescent protein expression of AT1 R SHRext significantly below SHRsed, and there is more significant difference(P < 0.05).3.4 WB test between groups in the SFO ACE2, AT1 and gp91 phox test valuesAfter eight weeks of swimming after the intervention, SHRsed group of ACE2 in SHRext group was significantly higher than that in the more significant difference(P <0.05);SHRext AT1 R content is significantly lower than SHRsed of group, and there is special significance difference(P < 0.01);Group SHRsed gp91 phox levels significantly higher than that of SHRext group, and there is a particularly significant differences(P <0.01) and more significant difference(P < 0.05)4. ConclusionLong-term aerobic exercise can effectively inhibit the SHR blood pressure with age growth and sympathetic nerve excitability, and these changes with the SFO AT1 R and gp91 phox reduced, increased ACE2...