| Objective This study from the perspective of aerobic capacity analysis of repeated intensive eccentric exercise effect mechanism of the mitochondrial changes in aerobic capacity, evaluation of rat skeletal muscle after motion micro damage degree and the body’s metabolism ability, discusses intensive repeated eccentric exercise on free radical metabolism and micro damage of skeletal muscle mitochondria activity of antioxidant enzymes and the effect of reveal repeated movement exists in the process of the change of mitochondrial energy metabolism system, for the prevention, diagnosis of exercise-induced skeletal muscle damage to provide scientific and accurate basis. Method Choose 72 adult male Wistar rats, were randomly divided into 3 groups: normal control group(K = 6), A big strength eccentric exercise group A(N = 30), A week after repeated movement group B(N = 30). Movement scheme to run downhill in rats, the speed of 25 m/min, slope- 16 degrees, movement time to rest 5 min after 30 min, 30 min in the sports. The movement time, a total of 60 min. One week after repeated movement in a movement to movement(a total of 2 times), the control group without any movement. Respectively in a large eccentric exercise intensity and repeat after a week immediately after exercise(0), 1 d and 2 d, 3 d, 7 d death of rats with cervical dislocation method and limbs of rats were taken of the quadriceps. Result⑴CK:After exercise group A 0 h, CK higher than the control group significantly(P < 0.01), 1-2 after motion increases to the peak, then gradually decline, and 7 d activity before return to sports level; Group B compared with group A CK activity trends are basically identical, but after 0 h, 1 d, 3 d enzyme activity is significantly lower than that of group A.⑵SOD:After exercise group A 0 h, SOD extremely significant decline in the CON group(P < 0.01), after 1-2 d fell to a minimum, then gradually rise, and 7 d activity level before return to sports. Group B compared with group A SOD activity trends are basically identical, but after 0 h, 1 d, 3 d enzyme activity is significantly higher than that of group A..⑶SDH:After exercise group A 0 h, SDH CON group significantly increased(P > 0.05), and then decreased, after exercise 2 d up to the peak, then fell back to levels before motion, motion after 7 d continue to decrease the CON group. Group B compared with group A SDH activity trends are basically identical, but after 0 h, 1 d enzyme activity was significantly higher than that of group A.⑷MDA:After exercise group A 0 h, MDA significantly elevated in the CON group(P < 0.01), 1 to 2 d after motion increases to the peak, then gradually decline, to 7 d after motion activity level before return to sports. Group B compared with group A SDH activity trends are basically identical, but after 0 h, 1 d, 3 d enzyme activity is significantly less than group A.⑸LDH:Similar to CK enzyme activity change trend, the overall upward trend is slow. After exercise group A 0 h, LDH compared with CON group significantly increased(P < 0.01), 1 to 2 d after motion increases to the peak, then gradually decline, to 7 d after motion activity level before return to sports. Group B compared with group A LDH activity trends are basically identical, but 0 h, 1 d and 2 d, 3 d enzyme activity significantly lower than that of group A.⑹GSH-PX:After exercise group A 0 h, GSH-PX compared with CON group significantly decreased(P < 0.01), after 1-2 d continue to drop to the minimum, 3 d increases to the peak, then fell to levels before motion, motion after 7 d levels significantly less than control group(P < 0.01). Group B compared with group A gsh-px activity trends are basically identical, but 0 h, 1 d and 2 d enzyme activity significantly higher than that of group A. Conclusion⑴ Group A after A workout can cause rat mitochondria oxidative stress state change.⑵ Group B after exercise also causes mitochondrial oxidatie stress state changes in rats, but CK, LDH and MDA activity was significantly lower in group A, tip: repetitive movements cause oxidative phosphorylation function improved, sports fatigue and reduce injury, the movement.⑶ Group B after exercise, SDH, SOD, gsh-px activity was significantly greater than in group A, tip: repeat intensive eccentric exercise can improve the ability of aerobic metabolism of skeletal muscle mitochondria and antioxidant capacity, to strengthen the body’s aerobic exercise ability.⑷ Group B after exercise and reduce the production of free radicals, improve the antioxidant enzyme activity, enhanced the SOD, gsh-px antioxidant enzymes the ability of scavenging free radicals, tip: repeat movement is advantageous to the skeletal muscle injury repair. |
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