MiR-192-5p Inhibites Proliferation And Migration Of Gastric Carcinoma Cells By Targeting BCL2

BACKGROUDGastric cancer(GC) is one of the most common malignant tumors in the world, because of its high morbidity, mortality and recurrence.However, the definite etiology and pathogenesis of GC are not clear yet.GC is difficult to be discovered at early stage, and occult metastasis leads to a poor outcome. Effective therapeutic targets and diagnosis depend on further understanding the molecular mechanisms of GC development and progression.Through comparative microRNA profiling, we found miR-192-5p is higher expressed in GC stomach tissues than health controls. In this study,we attempted to observe the effect of miR-192-5p on the proliferation of GC cell and the associated molecular mechanisms. Our data may imply a new sight into carcinogenesis and metastasis of GC.METHODS1. The expression of miR-192-5p in human GC mucus was detected by gene chip, and six human gastric cell lines(AGS, BGC-823, KATO-Ш,MGC-803, SGC-7901, GES-1) were detected by qPCR.2. LV3(H1/GFP&Puro)-hsa-mi R-192-5p inhibitor lentiviral vector was constructed and transfected into GC cell lines to screen stablelow-expression of miR-192-5p GC cells.3. Stable low-expression of miR-192-5p of AGS and SGC-7901 cells were screened by puromycin, miR-192-5p mimic was transfected into AGS and SGC-7901 at the same time. Then cell growth was detected by CCK-8Kit;cell migration was detected by Transwell assay.4. As predicted by PicTar and miRTarbase, BCL2 may be a target molecule of miR-192-5p. The effect of miR-192-5p on BCL2 was assessed by luciferase reporter assay. BCL2 was detected in low-expression or over-expression of miR-192-5p AGS and SGC-7901 cells by western blot.RESULTS1. miR-192-5p was higher in gastric cancer cell lines SGC-7901,BGC-823, MGC-803, KATO-Ш and AGS than normal human gastric epithelial cell line GES-1.2. LV3(H1/GFP&Puro)-hsa-mi R-192-5p inhibitor lentiviral vector was successfully constructed and stable low-expression of miR-192-5p of monoclonal SGC-7901 and AGS cells were successfully screened.3. After miR-192-5p was inhibited by hsa-miR-192-5p, the proliferation and cell migration of SGC-7901 and AGS cells were increased significantly. Conversely, cell proliferation was inhibited by mi R-192-5p mimic.4. When miR-192-5p was inhibited in GC cell lines, BCL2 was significantly elevated. Further, BCL2 was shown to be a target of miR-192-5p by luciferase reporter assay.CONCLUSIONSmi R-192-5p is expressed significantly higher in human GC cellsthan normal human gastric epithelial cells. miR-192-5p could inhibit proliferation and migration of GC cells through down-regulation of BCL2.