The α1-adrenoceptor Is Expressed On The Interstitial Cells Of Cajal And Its Possible Physiological Function In The Guinea Pig Prostate

Objective:The expression of α1-adrenoceptor was observed in the guinea pig prostate by double-labeling immunofluorescence staining from both the perspective of histology and cytology,to explore the expression of α1-adrenergic receptor on the interstitial cells of Cajal and its p athophysiological significant for further studying benign prostatic hyperplasia、chronic prostatitis、premature ejaculation and so on,which associate with the dysfunction of p rostate smooth muscle.Methods: T he prostate of adult guinea pigs as the research object in t his research. The guinea pig prostate frozen slices and cells culture were made for double-labeling immunofluorescence staining. c-Kit was used to distinguish ICCs,α-actin was used to label smooth muscle cells. cKit and α-actin were respective double- labeld with immunofluorescence stainingResults:The ICCs of c-Kit-positive cells were observed d istribution between smooth muscle in g uinea pigs prostate tissue sectioning a fter double-labeling immunofluorescence staining,on the ICCs,both c-Kit and α1-adrenergic receptor were positive,c-Kit-positive cells were observed in smooth muscle cells(SMCs). On the ICCs,The staining degree was higher with c-Kit than with α1-adrenergic receptor. T he prostate of ICCs of vitro cultivation were fusiform or star d istribution between smooth muscle and smaller than smooth muscle cells, ICCs c onnected with ICCs or SMCs by both sides of the bulge and displayed n etwork. T he prostate of ICCs of vitro cultivation using double-labeling immunofluorescence staining, then p laced under observation confocal microscope, both c-Kit and α1-adrenergic receptor were positive on the ICCs. F urthermore, v itro cultured prostate ICCs were minority in culture d cell s, t he vast majority cells were prostate SMCs, prostate SMCs were biger than prostate ICCs, the m orphological of prostate SMCs was diversity, e asily identification with ICCs cell under inverted microscope and most together with lumps.Conclusion:(1) The prostate ICCs really exist the α1-adrenergic receptor.(2) On the prostate ICCs, The staining degree of α1-adrenergic receptor was higher than the prostate SMCs.(3) The prostate ICCs and prostate SMCs can made a s ignal transduction system witch called “Neural signals- ICCs- smooth muscle” may be involved in the regulation of ejaculation process, the s pecific mechanism needs further research.(4) The three different forms of prostate ICCs witch s uccessfully isolated and cultured in vitro,probably three different subtypes of prostate ICCs and need further research.