The Expression Of CXCL12、CXCR4、PI-3K And AKT In Triple Negative Breast Cancer Cells Line

Background and Objective: Triple negative breast cancer is regarded as a special type of breast cancer that has fast development, highly aggressive behaviors and poor clinical prognosis. So far, it has not found an effective individualized treatment. Studying the mechanisms of invasion and metastasis in triple negative breast cancer is theoretical basis for the treatment triple negative breast cancer. In recent years, a large number of studies have shown that chemotactic factor CXCL12 and its receptor CXCR4 play an important role in the progress of tumor invasion and metastasis in a variety of carcinomas, included breast cancer.PI3K-AKT signaling pathway is an important signal transduction pathway, and associated with carcinogenic factor, its receptor and cell basic function.PI3K-AKT signaling pathway is a common abnormal activation signaling pathway in carcinoma. This study detect the expression of CXCL12、CXCR4、PI3K and AKT after transfection, analyze the relation of CXCL12-CXCR4 biological axis and PI3K-AKT signaling pathway in invasion and metastasis of triple negative breast cancer. Investigating the relevance of f CXCL12-CXCR4 biological axis and PI3K-AKT signaling pathway in triple negative breast cancer.Materials And Methods: We cultured triple negative breast cancer cells MDA-MB-231. We using pc DNA3.1 CXCR4 plasmid and si RNA-CXCR4 transfected human breast cancer cell line MDA-MB-231, respectively. After transfection, we using RT-PCR and western blot detected the m RNA and protein expression lever of MMP2CXCL12、CXCR4、PI3K and AKT in human breast cancer. Wound healing detect the ability of cell migration after transfection.MTT detect the ability of cell proliferation after transfection. SPSS 17.0 software is applied for statistical analysis. Between-group apply single analysis of variance(ANOV), P<0.05 show that it has statistical significance.Results: After over-expression CXCR4, compared with blank control and negative control,the m RNA and protein expression levels of CXCR4、PI3K and AKT is elevated in positive control;however,the m RNA and protein expression levels of CXCL12 is decreased,the difference is statistically significant(P < 0.05). After silent CXCR4, compared with blank control and negative control, the m RNA and protein expression levels of CXCR4、PI3K and AKT is decreased in positive control;however, the m RNA and protein expression levels of CXCL12 is elevated,the difference is statistically significant(P<0.05). After over-expression CXCR4, compared with blank control and negative control, the ability of cell migration and proliferation is elevated, The difference is statistically significant(P<0.05); however, After silent CXCR4, compared with blank control and negative control, the ability of cell migration and proliferation is decreased,the difference is statistically significant(P<0.05).Conclusion : 1. Chemokine receptors CXCR4 can feedback regulation the expression of chemokine factor CXCL12 in triple negative breast cancer. 2. Chemokine receptors CXCR4 can regulate the expression of PI3K-AKT signaling pathway. 3. CXCL12-CXCR4 biological axis can promote the migration of triple negative breast cancer via PI3K-AKT signaling pathway.