Relationship Between LPA Gene, PAI-1 Gene Polymorphisms And Patients With Primary Nephrotic Syndrome

Objective: Compare the levels of serum lipoprotein(a) [Lp(a)] and plasminogen activator inhibitor-1(PAI-1) in patients with primary nephrotic syndrome(PNS) with normal controls. To explore the relationship of three polymorphisms, 4G/5G(rs587776796) in the promoter of PAI-1 gene and +93C/T(rs1853021), +121G/A(rs1800769) of LPA gene,with the susceptibility to PNS.Methods: PNS patients and healthy controls were enrolled. The serum and blood samples from patients with PNS and healthy people were collected. Enzyme-linked immunosorbent assay(Elisa) was used to test PAI-1 levels in serum. Automatic biochemical analyzer detected the levels of serum Lp(a) and blood lipids, and collected their common clinical data. Extracted blood genomic DNA, then detected genotypes of all the participants by allele-specific polymerase chain reaction(AS-PCR) and DNA sequencing to explore the distributions of genotype frequencies and allele frequencies.Results: Compared with the NC group, the levels of PAI-1 and Lp(a) in serum were significantly higher in PNS patients. Both the genotype frequencies and the allele distributions of 4G/5G polymorphism were significant difference between the two groups.The serum level of PAI-1 was significant higher in patients with 4G/4G genotype than5G/5G genotype. The distribution of genotypes includes(CC, CT, TT) in LPA+93C/T polymorphism was no significant difference between PNS and NC subjects, but the difference was found in CC versus CC+TT genotypes. And the frequency distributions of allele C/T was different between the two groups. No significant difference of serum Lp(a)level was detected among the three genotypes of LPA+93C/T, and the the same result was found among the three genotypes of LPA+121A/G polymorphism. Meanwhile, the genotypes and allele frequency distributions of LPA+121A/G polymorphism were not statistically difference between PNS and NC groups.Conclusions: The increased levels of Lp(a) and PAI-1 in serum are two risk factors of PNS. Polymorphisms of 4G/5G in PAI-1 gene and +93C/T in LPA gene are associated with the susceptibility of PNS. No significant association is found between LPA+121A/G polymorphism and the risk of PNS.