Establishment Of The Animal Model For The Study Of The Mechanism Of Urinary Tract Epithelial Repair After 2 Micron Laser Resection Of Prostate

Objective: To establish a scientific, feasible and reproducible animal model for the study of the mechanism of the urethra epithelial repair after Two-micron laser resection of prostate.Methods: There were a total of 45 male Chinese pastoral dogs. The screening method is as follows: 5 years of age or older, weight more than 11 kg, normal testis morphology, rectal examination indicate prostate volume increased significantly.There are 15 dogs in each group. Established three animal operation model:Two-micron laser vaporization resection of the prostate Via the bladder(group Tm RLP),Two-micron laser vaporization resection of the prostate add part of mucosal of bladder neck Via the bladder(group Tm RLPB), Two-micron laser vaporization resection of the prostate Via the bladder add bilateral ureteral skin fistulization(urinary diversion)(group Tm RLPU). The age and weight of the dog were recorded before operation.The operation time and the recovery time of anesthesia were recorded.After operation, we need to observe the canine survival and the three groups of dogs’ prostatic urethra wound repair process and the Tm RLPB groups of dogs,bladder neck wound repair process respectively after 3 days, 1 week, 2 weeks, 3weeks, 4 weeks under cystoscope. And then,the prostate and bladder intact tissue was surgically removed.The head and tail diameter,transverse diameter,and dorsal ventral diameter of prostate(Three diameter of prostate) was measured. HE staining was performed to observe the morphological changes in the morphology of the prostate of the three groups of dogs and the changes of the morphology of the bladder neck of the Tm RLPB group.Results: In this study, the male Chinese pastoral dogs.of the age of 5-7 years old,weight 18-22 Kg, testicular development is normal,and the anus refers to the volume of the prostate gland increased significantly were chosen.Three diameter product of the dog prostate were: 35.43 + 8.36cm3, 41.23 + 10.45cm3, 44.56 + 11.24cm3 in Tm RLP group, Tm RLPB group,and Tm RLPU group.They are all greater than 18cm3.In this study, 45 dogs were successfully completed surgery, of which 41 experimental dogs survived until the line is expected to check and 4 dogs were not expected to die.The overall survival rate was 91%. After 3 days, 1 weeks, 2 weeks, 3 weeks, 4 weeks,Tm RLPB group, Tm RLP group, Tm RLPU group, the survival rates were respectively100%、93%、93%、93%、93%,93%、93%、93%、93%、93%,93%、93%、86%、86%、86%.1 dogs died fifth days after operation in Tm RLP group;1 dogs died second days after operation in Tm RLPB group. 2 dogs respectively died 3H and 10 days after operation in Tm RLPU group. Postoperative cystoscopy and microscope observation results showed that: in Tm RLP group,the prostatic urethra wound re-epithelialization is from the added coverage of residual prostate tissue. The start and finish time was 1 week and 3 weeks after the operation respectively.Tm RLPB group and Tm RLP group prostate ministry urethral wound re-epithelialization presents the same source of tissue and time course, but the origin and time course of bladder neck wound re-epithelializationoriginprostate was different from the prostate ministry urethral.The performance was that invasived margin of normal bladder neck urinary tract epithelial crawling coverage.The re-epithelialization start time was 2weeks after operation.It had not completed 4 weeks postoperatively. There was the same prostatic urethra wound re-epithelial origin between the Tm RLPB group for urinary diversion and the Tm RLP group, but the start and finish time is later than Tm RLP group, the prostatic urethra wound re-epitheliathe of the Tm RLPB group starting from 2 weeks after operation.It had not completed 4 weeks postoperativelyConclusion:The animal models of canine Tm RLP, Tm RLPB, Tm RLPU who is successfully established.They respectively reflect the process of the prostatic urethra epithelial,s "re-epithelialization" from the different factors and angles such as the source of repair, repair method and influence factor after prostatectomy. It is scientific, feasibility and repeatability. It is an ideal animal model for in-depth study for the study of the mechanism of the urethra epithelial repair after Two-micron laser resection of prostate.