The Preparation And Decoration Of 3-Aminophenol Formaldehyde Resin Microspheres And Their Applications In Carcinoembryonic Antigen Detection

Phenolic resin (PR) materials have been applied in the biomedical filed such as cell delivery, targeting and imaging because of their good biocompatibility and low toxicity. Monodispersed spherical PFR materials have several additional properties like facile preparation method, multiple-choice of composition and flexible size control, which render them to be the basic of many complex constructions. This dissertation mainly investigates the controllable preparation of 3-aminophenol formaldehyde resin (3-AFR) microsphere, the construction of 3-AFR based Surface-enhanced Raman scattering (SERS) substrate and the application of biomarker carcinoembryonic antigen (CEA) detection.The major contributions are as follows:1. Firstly,3-AFR microspheres with uniform size distribution and regular morphology were prepared by the route of sol-gel. With the mixture of ethanol and water as solvent, the polycondensation and polymerization of 3-aminophenol and formaldehyde produced resin sol and gel under the catalysis of ammonium hydroxide. The factors influencing the size of as-prepared resin sphere have been discussed including the amount of catalyst ammonium hydroxide, the amount of precursor 3-aminophenol and formaldehyde and the ratio of ethanol and water. Adjusting these factors the controllable preparation has been realized. We also explained the principle of the preparation method and characterized the active groups on the surface of the 3-AFR microsphere.2. Secondly, using facile water bath approach silver nanoparticles (AgNPs) have been in-situ reduced and immobilized on the surface of the 3-AFR microsphere. Their abundant reductive groups can easily transform the ionic silver (Ag+) into elemental silver (Ag0). The continuous reducing of Ag+ formed dense Ag layer outside of the 3-AFR microsphere. The as-prepared AgNPs/3-AFR microsphere compound was used to work as SERS substrate. Subsequently Raman signal molecules 4-Mercaptobenzoic acid (4-MBA), Rhodamine 6G (R6G) and Nile blue A (NBA) were detected and the enhancement effect of the SERS substrate was evaluated.3. Finally, the AgNPs/3-AFR microsphere compound SERS substrate was used to identify and quantitatively detect biomarker CEA. After correspondingly connecting the CEA labeling antibody and capture antibody with the NBA absorbed AgNPs/3-AFR microsphere and the magnetic nanoparticle, CEA would specially recognize and conjugate forming sandwich like compound. Collect the compound by the imposed magnetic field and detect the Raman signal to fulfill the identification and quantitatively detection of biomarker CEA. The result showed a good linear correlation between the detected NBA signal and the concentration of CEA and the detection limit was estimated to be 1.2 pg/mL.