Preparation And Evaluation Of Resveratrol Nano/Micro Carriers

Resveratrol is an effective anti-ageing drug. In addition, it also has antioxidant capacity and whitening effect. However these potential applications of resveratrol in cosmetic and pharmaceutical are restricted by its poor water solubility and light instability. Therefore, the aim of this study was to develop a delivery system for potential use of resveratrol. Lipid based nanocarriers with different lipid compositions were prepared and characterized to investigate factors influencing skin targeting potential of lipid nanocarriers.Resveratrol-loaded nanostructured lipid carrier (NLC) was produced using hot high-pressure homogenization method (HPH). In present study, solubility study and miscibility study were used to screen the matrix lipids. The combination of emulsifying ability, particle size, PDI and stability was used to screen the optimum type and concentration of surfactant. Finally, single factor method was conducted to determine the process parameters of HPH.The particle size, zeta potential, structure and morphology of Resveratrol-loaded NLC were characterized. Physical stability study suggested that Resveratrol-loaded NLC had good physical stability. Resveratrol-loaded NLC protected resveratrol against UV-light. In vitro antioxidant activity study showed an obvious radical scavenging activity of Resveratrol-loaded NLC. The result of release test proved that the release mechanism of Resveratrol-loaded NLC conformed to Weibull equation.In present study, resveratrol-loaded lipid nanocarriers with different lipid compositions were developed. From physicochemical properties and comparative evaluation, it is clearly suggested that the lipid nanocarriers made from different lipid compositions possess different characteristics. When coupled with the values of entrapment efficiency and particle size after 6 weeks of storage, the nanocarriers with higher proportion of liquid lipid had better physical stability. By controlling the crystallisation of lipid nanoparticles, the release rate at the burst release phase could be modulated. Since particle size, crystallization, and release profile were affected by the composition of lipid matrix, the retention amounts of resveratrol in the skin were also related to the lipid composition. The nanocarriers with low liquid lipid ratio were considered to be more potential for topical application due to the advantage of skin absorption efficacy.