The Injury Of Low-Intensity Extracorporeal Shock Wave On Rabbit’s Penile Tissue

Objective: To observe the low- intensity extracorporeal shock wave on rabbit penile tissue injury and repair, and to investigate the security of LESW therapy on the erectile dysfunction(ED). Methods: Common adult male rabbits(n=60) were randomly divided into 6 groups(5 experimental group, 1control group). Same dose of LESW(E=0.09 m J/mm2,60shocks/min,500 shocks each time on each side) treat on corpus cavernosum. Animals’ penile tissue are observed the existence of the corresponding injury. Corpus cavernosum were made pathological examination, observation under light microscope HE: leukocyte, hemangiectasis, hemorrhage; Masson: intracavernous collagen fibers; observation under electron microscope :smooth muscle cell, collagen, capillaries. Penile tissue homogenate SOD activity was evaluated by xanthine oxidase method, thiobartituric acid method to detect the contents of MDA. Results: During the treatment, all rabbits have no penile superficial tissue injury occurred. HE staining microscope showed no inflammatory cell infiltration, outside the capillaries no overflow of red dlood cells. All experimental group the corpus cavernosum capillaries expansion. No significant differences between the experimental group. There is no obvious hyperplasia of collagen fibers in Masson staining. Electron microscope showed : in group D,smooth muscle cells swelling, between cells, collagen increased obviously, capillary stracture incomplete. The content of MDA and SOD,group D compared with group F, p<0.05, the difference was statistically significant. MDA content in D group was obviously higher than that of group F, SOD content lower.Group A、B、C、E compared with F,p>0.05,there is no statistically significant. Conclution:Low-energy extracorporeal shock wave can promote the penile tissue vascular endothelium expansion. There is no obvious collagen fibers deposition. Low- intensity extracorporeal shock wave for the treatment of penile tissue two weeks continuous organization has no obvious injury to the penis, interval of 3 days of continuous treatment of penile tissue injury cannot be completely repaired.