Synthesis And Fluorescence Probe As Nuclear Targeting By Novel Ruthenium(Ⅱ) Complexes Coordinated With CQM/DPPZ

At present, the fluorescence imaging is widely used in biochemistry, molecular biology and clinical diagnosis as a microscopic observation technology and it is commom method for observed the distribution and intensity of the fluorescent material inside the cells. To help develop its full potential, optimized imaging systems and fluorescent contrast agents will be needed. Although many organic dyes have been designed and investigated, the utilization of these organic dyes is limited because of their poor water solubilities, low photo-stabilities, and high toxicities. To overcome the drawbacks of organic dyes, transition metal complexes, especially versatile ruthenium(Ⅱ) complexes, have increasingly attracted attention as potential fuorescent probes because of their wide spectral range, long-term luminescence, red emission, large Stokes shifts and good water solubility. Increasing evidence found that Z-DNA closely related to gene transcription and gene regulation. Besides, Z-DNA is an instable DNA conformation in vivo and now the compounds can effectively induce the formation of B-DNA to Z-DNA are still rarely reported. Ruthenium complexes with dipyridophenazine r(DPPZ) ligands/phenazine(CQM) were synthesized under microwave irradiation, and their properties for imaging of the nuclei of living tumor cells and DNA conformation transition were investigated.The contents of the thesis are carried out as following:1. Ruthenium(Ⅱ) complexes with dipyridophenazine(DPPZ) ligands/phenazine(CQM) were synthesized under microwave irradiation. The stuctures of these complexes were characterized by ESI-MS, 1H NMR and 13 C NMR and research resu lts agree well wit h the theoretical values.2. Ruthenium(Ⅱ) complexes with phenazine(CQM) have good photophysical properties, strong fluorescence intensity and fluorescence quantum yield large. The cellular uptake and distributions of complexes confirmed using confocal laser scanning microscopy. The results showed that both complexes were taken up by cells which was localized in the cell nuclei. Low cytotoxicity against the growth of various cells for both complexes was confirmed using an MTT assay. Further real-time fluorescence and UV-vis absorption spectra showed that target complexes can quickly and efficiently uptake into the cell. Several spectroscopy mothods were used to determine the interaction between target complexes and CT-DNA/BSA, and the results show that the target complexes were binding to BSA stronger than CT-DNA.3. Electronic Absorption Spectrum, Electron Emission Spectrum, CD Spectrum have been used to research on the molecular recognition between ruthenium(Ⅱ) complexes with c-myc G4 DNA. The results show that the target complexes can induce info mation and stable the structure of c-myc G4 DNA.