Effect Of Drying On Ginkgolic Acids In Ginkgo And Observation Of The Effect Of Ginkgols And Cisplatin On Tumor

Ginkgolic acids and ginkgols are alkylphenol compounds and extensively exist in leave, nut and sarcotesta of Ginkgo biloba L. Ginkgolic acids are considered to be the main allergic substance in Ginkgo biloba. Therefore, in the process of ginkgo nut, in order to achieve the requirement of food safety, it should control and reduce the alkylphenol compounds. At the same time, this compound show prominent bactericidal, insecticidal and antitumor activity. It has great applicable potential in regard to the development of plant pesticide and the food and drug industry.In this paper, firstly, dry processing of ginkgo nut were studied, and the effect of different drying methods on the content of ginkgo acids and ginkgols were tested. Secondly, the effect of ginkgols, which a degradation product of is the ginkgolic acids, combined of cisplatin in the treatment of H22 tumor-bearing mice. And the antitumor preliminary mechanism of ginkgols was explored. The main conclusions summarized as follows:1. Ginkgo nut was dried by microwave and infrared drying methods, which of60? C drying treatment as control. The contents of ginkgolic acids and ginkgols in dried ginkgo nut with different drying methods were compared. The results showed that contents of ginkgolic acids and ginkgols in ginkgo nut which dried with 700 W power of microwave or infrared temperature 80?C were slightly lower than that of60?C dried sample. If ginkgo nut power was treated with certain amount of Na2CO3 for 2-12 h under the same drying condition, the contents of ginkgolic acids and ginkgols decreased obviously and the reduction ratio of which had not showed dependence on the alkaline processing time. Compared with the 80 ? C infrared drying after alkali treatment, the content of ginkgolic acids was reduced 39 % and ginkgols was reduced 23.3 % with 700 W microwave alkaline drying.2. The effect of ginkgols on migration, invasio of HepG2 cell was studied. The MTT assay was used to test viability rate of HepG2, SW480, HGC cells treatment with ginkgols combined cisplatin. The effect of ginkgols on migration, invasion abilities of HepG2, SW480 cell was detected by Transwell assay. The effect of ginkgols on expression of MMP-7, Rho A and P-AKT protein was detected by westernblot technology test. The results showed that ginkgols had significant suppressive effect on HepG2, SW480, HGC cells growth in the concentration range of 0-80 μg/mL. When the concentration ratios of ginkgols and cisplatin were at 2:1, 4:1 and 8:1, the ginkgols and cisplatin showed synergetic inhibited proliferation effect. Synergy indexes were 1.23, 1.19, 1.16, respectively.The migration and invasion abilities of the HepG2, SW480 cells that treated with ginkgols at the concentration of 20, 40, 80 μg/m L for 24 h were gradually diminished.The HepG2, SW480 cells were treated with different concentrations of ginkgols for24 h. with the concentration of ginkgols increased, it observed that the expression of MMP-7, Rho A and P-AKT decreased significantly.3. The effect of ginkgols combined cisplatin on the growth of transplanted tumor in mice. H22 hepatocarcinoma xenograft models were established to be randomly divided into the model group, cisplatin group(7.5 mg/kg), half dose cisplatin group(3.75 mg/kg), ginkgols group(40 mg/kg), half dose cisplatin(3.75 mg/kg) plus low(10 mg/kg), middle(20 mg/kg) and high(40 mg/kg) dose ginkgols group. And daily administration of intraperitonal injection for 10 days. Blood was collected and mice were killed. The results showed that antitumous effect of 40 mg/kg ginkgols combined half dose cisplatin(3.75 mg/kg) was similar to cisplatin group(7.5 mg/kg and superior to half cisplatin group(3.75 mg/kg), reduced the toxic effect of cisplatin,avoided weight loss in mice. Q value was equal to 1.46 which is greater than 1.15, so40 mg/kg ginkgols could improve the suppression ability of 3.75 mg/kg cisplatin on tumor. Compared with 3.75 mg/kg cisplatin group, the combination of half cisplatin and ginkgols enhaced the spleen, thymus index. 40 mg/kg ginkgols could inhibit the growth of transplanted tumor in mice and add positive effect to cisplatin.4. Tumor-inhibitory role of ginkgols in cisplatin chemotherapy to Heps hepatocarcinoma xenografts and its effect on serum levels of IFN-γ、IL-2 and TNF-α.Heps hepatocarcinoma xenograft models were established to be randomly divided into the model group, cisplatin group(7.5 mg/kg), ginkgols group(40 mg/kg),cisplatin(7.5 mg/kg) plus low(20 mg/kg), high(40 mg/kg) ginkgols group. And daily administration of intraperitonal injection for 10 days. blood was collected and micewere killed. The results showed ginkgols group(40 mg/kg) plus cisplatin not only enhance the thymus, spleen and liver index, as well as the level of IFN-γ、IL-2 and TNF-α. Q value was equal to 1.16 which is greater than 1.15, so 40 mg/kg ginkgols could improve the suppression ability of 3.75 mg/kg cisplatin on tumor. 40 mg/kg Ginkgols could effectively inhibit hepatocellular carcinoma in vivo via enhancement of host immune system function to increase the capability of antitumor of cisplatin.