The Effect Of MiR-224 In Cervical Cancer SiHa Cell Proliferation, Invasion And Apoptosis

Objective:To investigate the effect of mi RNA-224 in cervical cancer Si Ha cell proliferation,apoptosis and invasion.Methods:To overexpression and inhibition the expression of mi RNA-224 in human cervical cancer cell lines(Si Ha),Si Ha was transient transfected with mi RNA-224 mimics or mi RNA-224 inhibitor.The Si Ha cells were divided into experimental group(mi RNA-224 mimics and mi RNA-224 inhibitor,E),negative control group( Negative control,mi RNA-224 inhibitor NC,NC) and blank control group( BC).Then q-PCR(quantitative real-time polymerase chain reaction) was used to detect the expression of mi RNA-224 in each group.When Si Ha cell was successfully transfected,CCK-8 method,wound healing test,flow cytometry technique were used to detect the change of cellular proliferation,invasion and apoptosis separately.Results:1.Overexpression of mi RNA-224 could promote Si Ha proliferation and cell scratch healing,inhibition of Si Ha cell apoptosis.2.Silencing the expression of mi RNA-224 can reduce Si Ha proliferative and inhibit cell scratch healing,promote Si Ha cell apoptosis.Conclusion:1.Upregulating the expression of mi RNA-224 could promote proliferation,migration and inhibitor apoptosis of Si Ha cell;2.Silencing the expression of mi RNA-224 could suppress the proliferation,migration and promote apoptosis of Si Ha cell.