The Role Of Adiponectin In The Emphysema Rats With Osteoporosis Induced By Different Ways Of Rearing And Tobacco Smoke

Objective:By observing the degree of emphysema and osteoporosis, and the serum tumor necrosis factor(TNF)-α, interleukin(IL)-6 and adiponectin(APN) levels of rats, which were fed with normal diet, fat-rich diet or limited intake diet and exposed to tobacco smoke or room air, we aim to explore the role of APN in the process of emphysema rats with osteoporosis induced by different ways of rearing and tobacco smoke.Methods:Forty-eight 6-week clean male SD rats were randomly divded into normal diet +smoking group, fat-rich diet+smoking group, limited intake + smoking group, normal diet group, fat-rich diet group, and limited intake group, which were exposed to tobacco smoke or room air and fed with corresponding diet. The relevant indices were measured in each groups after 6 months. Lee’s index was calculated. Mean linear intercept(MLI) 、 mean alveoli number(MAN) 、 Trabecular Bone area ratio(Tb.Ar) 、 the number of bone trabeculae(Tb.N) and the bone trabecula space(Tb.Sp)were calculated by the microscope.The serum TNF-α、IL-6、APN and osteocalcin(OC) levels were detected by ELISA kits.Results:1.smoking made rats’ Lee’s index decreasing. Both in non-smoking group and smoking rats, Lee’s index in fat-rich diet group was the highest, and limited intake group’Lee’s index was the least. The differences were all significant(all P<0.05).2.Smoking made rats’ MLI increasing and MAN decreasing. In smoking rats,the MLI(102.71±11.29)um in limited intake group was the highest and MAN(130.85±19.8)/mm2 was the lowest, and fat-rich diet group’ MLI was the lowest and MAN was the highest,the differences were all significant(all P<0.05).3.Smoking made rats’ Tb.Ar 、 Tb.N 、 OC decreasing and Tb.Sp increasing. In smoking rats, Tb.Ar(14.23±1.08)%、Tb.N(3.04±0.30)/mm2 and OC(296.15±2.81)mg/L in fat-rich diet group were the highest, Tb.Sp(270.66±30.33) m g/L was the lowest;Tb.Ar(7.96±1.41)%、Tb.N(2.11±0.25)/mm2 and OC(275.72±3.44)mg/L in limited intake group were the lowest, Tb.Sp(445.05±28.17)mm was the highest,the differences were all significant(all P<0.05).4.Smoking made rats’ TNF-a and IL-6 lenels increasing. In smoking rats, Serum TNF-α and IL-6 levels in limited intake group and fat-rich diet group were higher than those in normal diet group. The differences were all significant(P<0.05).5.Serm APN level in normal diet group and limited intake group was higher than Corresponding non-smoking rats. In smoking rats,serm APN level was the highest in limited intake group(9.60±0.96)mg/L,and was the least in fat-rich diet group(4.23±0.51).The differences were all significant(P<0.05).6.Indicators which reflects the extent of emphysema and the indicators of bone mass are relevant. The reduction of bone mass was positively correlated with emphysema.7.In both smoking and non-smoking fat-rich diet group, APN levels had a negative correlation with the degree of emphysema and the level of inflammatory factors.In both normal diet and limited intake smoking rats, APN lenels had a positive correlation with the degree of emphysema and the level of inflammatory factors.Conclusions:Different ways of rearing and tobacco smoke both can influence the formation of emphysema and osteoporosis. There is a positive correlation between emphysema and osteoporosis. APN may paly an important role by regulating systemic inflammation.