Expression Of Endoplasmic Reticulum Associated Degradation Protein Derlin-1 In Early Cervical Cancer And The Relationship Between Derlin-1 And Clinical Or Pathological Characteristics Of Cervical Cancer

Objective:To illuminate the role of Derlin-1 in the occurrence and development of cervical cancer and provide experimental basis for the research on the pathogenesis of cervical cancer by detecting the relationship between Derlin-1 and clinical or pathological characteristics of cervical cancer.Methods:49 samples of cervical cancer, 40 samples of cervical intraepithelial neoplasia(CIN)II-III and 30 normal controls from patients who received operation at Sun Yat-sen Memorial Hospital, Sun Yat-sen University from March 2011 to May 2014 were collected.All cervical cancer patients without the second cancer merger or secondary didn’t have chemotherapy, radiotherapy or biological therapy before operation and had complete clinical data. Cervical cancer was staged according to the staging criteria of the International Federation of Gynecology and Obstetrics(FIGO) in 2009. The tumor covered more than 50% of the wax confirmed by conventional histopathologic sections. There is no hemorrhage or necrosis in the tumor and no inflammation in surrounding tissues. The expression level of Derlin-1 was detected by immunohistochemistry and the infection of high risk human papillomavirus(HPV) was identified by HC2. Data were statistically processed by SPSS software to analyze the expression level of Derlin-1 in early stage cervical cancer and its relationship with clinical or pathologic features in cervical cancer.Results:In the experiment, Derlin-1 was mainly located in cytoplasm. The positive expression rates of Derlin-1 protein in cervical cancer, CIN and normal cervical tissue were respectively 55.1%(27/49), 32.5%(13/40) and 6.7%(2/30). The results showed that the expression level of Derlin-1 in cervical cancer was significantly higher than that of CIN(χ2=4.547, P=0.033) and normal cervix(χ2=18.789, P<0.001). The expression level of Derlin-1 in CIN group was significantly higher than that of normal cervix group(χ2=6.795,P=0.009). The expression level of Derlin-1 showed a trend of increase(ⅠB1+B2: 51.5%and ⅡA1+A2: 62.5%) as FIGO staging went up, but there was no significant difference. It was turned out that Derlin-1 had no obvious relationship with patients’ age, clinical stage, tissue differentiation degree, lymph node metastasis, vascular presence of tumor and clinical pathological types(P>0.05). However, compared with cervical cancer patients without HR-HPV infected, the positive rate of Derlin-1 in cervical cancer patients with HR-HPV infected was 64.9%(24/37) and the difference was statistically significant(P=0.016).Conclusions:Endoplasmic reticulum associated degradation protein Derlin-1 has high expression in patients with cervical cancer and the expression level of patients with high cervical intraepithelial lesion(CINII-III) is also higher than that of normal cervix. It is demonstrated that Derlin-1 plays an important role in the forming of cervical cancer. The high expression of Derlin-1 associated with high HR-HPV infection. However, we do not find Derlin-1 is associated with patients’ age, clinical stage, tissue differentiation degree,lymph node metastasis, vascular presence of tumor and clinical pathological types.