Experimental Study Of Glial Cells And Nerve Function Changes On Per-infarction By Electro-acupuncture

Objective:After cerebral infarction often left with varying degrees of neurological impairment,and therefore neurological rehabilitation and reconstruction is the focus of neuroscience research.Present study suggests that glial cells play an important role in neuronal regeneration and functional recovery of the nerve; and found that electro-acupuncture for neurological rehabilitation treatment have better results.Therefore, this article by observing the influence of electro-acupuncture at different points in time on a variety of glial cell marker expression and nerve function in rats after cerebral infarction,further discussion whether the mechanism of electro-acupuncture therapy improves neurological recovery be related to it could regulate proliferation and activation of astrocytes,oligodendrocytes and microglia.Methods:Used 2 ~ 3 months old, weighing 100 ~ 150 g, healthy male SD rats, to copy Stroke-prone renovascular hypertensive(RHRSP) rats.After bilateral renal artery stenosis,measured the tail arterial blood pressure once a week. Preparation RHRSP Model 10 weeks, took blood pressure ≥180 mm Hg, weighing 400 ~ 450 g, and no neurological deficit symptoms RHRSP rats, using electro-coagulation copy middle cerebral artery occlusion(MCAO) model.Digital randomization method:divided into sham operation(SM) group, cerebral infarction(CI) group and electro-acupuncture(EA) group.After10% chloral hydrate(250 mg/kg) intraperitoneal injection of anesthesia, RHRSP rats of CI group and EA group through the left temporal craniotomy, occluded middle cerebralartery(MCA) to copy MCAO model;Assessment the score of neurobehavioral changes after MCAO1 day, 3days, 5days and7 days by Garcia neurological score; Observation and comparison the infarct volume size after MCAO7 days, 14 days, 21 days and 28 days by TTC staining; Immunohistochemical observations the positive expression of glial fibrillary acidic protein(GFAP), myelin basic protein(MBP), ionized calcium binding adapter molecule 1(Iba1) in infarction and pre-infarction.Results:1. 119 rats were successful replicated to RHRSP model after 10 weeks of renal artery stenosis, and the average blood pressure was 193.46±17.39 mm Hg.2.The Garcia score was used to evaluate the nerve function of each rat, the SM group at all time comment points were normal(18 points); after MCAO, rats appear different degree of neural function defect, and continue to improve over time, the neural function score of EA group was significantly higher than that of CI group at 1 days, 3 days, 5 days and 7days time points, the difference between the groups has statistical significance(P < 0.05,P> 0.05). 3.TTC staining showed that the infarct size was significantly smaller than that of EA group at 7 days, 14 days and 21 days after MCAO, the difference of the groups was statistically significant(P < 0.05), and the difference at 28 days was not significant.4.After MCAO there was obvious proliferation of glial cells. The number of GFAP and Iba1 positive cells for CI group and EA group pre-infarction were higher than that in SM group, GFAP positive cells of EA group were lower than that in CI group at four time points, it is the most significant at 7 days(P < 0.05, P> 0.05);Each time points, the Iba1 positive cells of EA group decreased comparing with group CI, and the most obvious difference between two groups was 7 days(P < 0.05, P > 0.05);After MCAO the positive expression of MBP in infarction and pre-infarction was reducing, which the MBP positive expression of CI group and EA group in each time points were lower than SM group, and gradually returned to normal. positive expression of MBP in EA group at each time point were higher than in CI group, the difference between groups is statisticallysignificant(P< 0.05, P > 0.05).Conclusion:Electro-acupuncture treatment can improve the recovery of neurological function after cerebral infarction, and can reduce the volume of cerebral infarction.electro-acupuncture can inhibit the positive expression of MBP, and promote the positive expression of Iba1 and GFAP, which can be used to regulate the proliferation and activation of astrocytes, microglia and microglia, and the mechanism may be related to the improvement of nerve function recovery by electro-acupuncture.