| ObjectiveTo study the effects of miR-363-3p on the proliferation, apoptosis, invasion and migration of human gastric cancer cell lines SGC-7901, MKN-45 and MGC-803.Methods miR-363-3p sequence and the antisense sequence of miR-363-3p were transfected into human gastric cancer cell lines SGC-7901 and MKN-45, MGC-803 cells by lentiviral vector with fluorescent marker chemical synthesis, fluorescence microscope was used to observe the number of fluorescence in cells, at the same time application real-time fluorescence quantitative PCR(RT-PCR) to detect the expression of miR-363-3p among up-regulated and down- regulated group with the corresponding negative controls group. The influence of miR-363-3p on proliferation ability of three gastric cancer cell lines was detected by CCK-8 assay. The influence of miR-363-3p on cell lines apoptosis and cycle of three gastric cancer cell lines was detected by flow cytometry assay. The influence of miR-363-3p on invasion and migration ability of three gastric cancer cell lines was detected by scratch and Transwell assay.Results ① After transfection,the expression of miR-363-3p up-regulated group in SGC-7901,MGC-803 and MKN-45 cells increased 0.62,0.68,0.63 fold(p<0.05), while in down-regulated group decreased 58.2,78.6,84.2 fold(p<0.05), both compare to corresponding negative group in various strains of gastric cancer cells. ② The CCK-8 assay and flow cytometry assay showed that there was no significant difference on the proliferation, apoptosis rate and cell cycle of gastric cancer cell lines between miR-363-3p up-regulated group and negative control group after transfection(p> 0.05), there was no significant difference on the proliferation, apoptosis rate and cell cycle of gastric cancer cell lines between miR-363-3p down-regulated group and negative control group after transfection. ③The scratch assay showed that the healing degree of miR-363-3p down-regulated group had obvious difference compare to negative control group at the same time, especially at 48 h(p< 0.05), the healing degree of miR-363-3p down-regulated group more than the negative control group.The healing degree of miR-363-3p up-regulated group had obvious difference compare to negative control group at the same time, especially at 48 h(p< 0.05), the healing degree of miR-363-3p up-regulated group less than the negative control group. ④The Transwell migration and invasion assays showed that the number of migrating cells in miR-363-3p downregulated group had obvious difference compare to negative control group at 48 hours(p< 0.05), the number of migrating cells in miR-363-3p upregulated group more than the negative control group. The number of migrating cells in miR-363-3p downregulated group had obvious difference compare to negative control group at 48 hours(p< 0.05), the number of migrating cells in miR-363-3p up-regulated group less than the negative control group.ConclusionmiR-363-3p may be involved in the invasion and migration of gastric cancer. |
PDF Full Text Request