The Expression Of Snail, MYH9, GLIT9, FUT6 In Patients With Diabetic Nephropathy

Objective:Diabetic nephropathy is a significant cause of chronic kidney disease and end-stage renal failure worldwide, particularly in developed countries. 20%-30% of the patients with diabetic develop diabetic nephropathy. Type 2 diabetes constitutes about 85%–95% of all diabetes cases.The pathogenesis of diabetic nephropathy has not been clarified.Till now,it occurs as a result of interaction between both genetic and environmental factors in individuals. Genetic factors, glucose and lipid metabolism disorder, oxidative stress, Renal hemodynamic changes, podocytes injury,inflammation, epithelial mesenchymal transdifferentiation, and other factors are involved in the pathogenesis of diabetic nephropathy. Interventions such as glycaemic control, blood pressure control and inhibition of the renin–angiotensin– aldosterone system have been shown to slow this progression. Despite the implementation of these strategies, the number of patients with diabetes that ultimately develop end-stage renal disease remains high. Progress in the knowledge of pathogenesis of DN, optimization and identification of novel, potent anti-DN agents should pave the way for greater insight into the treatment of DN. Our preliminary study found rs735853,rs2269532,rs2071731,rs739097 SNP loci of MYH9 gene, rs7660895,rs1014290 SNP loci of GLUT9 gene, rsl526167, rsl7304270 SNP loci of TOX gene,rsl2102171 SNP loci of SMAD3 gene, rsl0811661 SNP loci of CDKN2A/B gene,rs4402960 SNP loci of IGF2BP2 gene are associated with diabetic nephropathy.To investigate the mechanism of the pathogenesis of diabetic nephropathy by detecting the expressions of Snail, MYH9, GLUT9, FUT6,HAAO in kidney biopsy of patients with type 2 diabetic nephropathy.Methods:Collected in August 2008- March 2013 in Tianjin Medical University General Hospital hospitalized patients with DN were confirmed by clinical and pathological analysis of renal tissue samples, thirty-two patients with DN who underwent renal biopsy were collected as DN group. Ten subjects without DN who underwent renalbiopsy were taken as control group. Patients informed consent of the experiment.In DNgroup,patients were accord with standard of DM and DN.All patients were accord with out standard of DN.Collect the patient’s general data including sex,age,weight,height,blood pressure,etc. linical and biochemical indexes, including Glu、TG、TC、LDL、HDL、BUN、Cr、UA、ALB、urine micro-albumin and urine protein in 24 h, etc. The expressions of Snail, MYH9, GLUT9,FUT6,HAAO in renal tissues were detected by immunohistochemistry stain.Results:(1) Snail were expressed in the renal tubular epithelial cell nucleus of both DN group and control group, while the expressions of Snail were significantly higher in DN group than those in control group.(2) MYH9 were expressed in glomerular podocytes and renal tubular in DN group, while it were not expressed in almost all parts of kidney in control group.(3) GLUT9 were expressed in the basolateral side of the proximal tubule of both DN group and control group, while the expressions of GLUT9 were significantly higher in DN group than those in control group.(4) FUT6 were expressed in the renal tubular epithelial cell nucleus of both DN group and control group, while the expressions of FUT6 were significantly higher in DN group than those in control group.(5) HAAO were expressed in the renal tubular epithelial cell cytoplasm of both DN group and control group, while the expressions of Snail were significantly higher in DN group than those in control groupConclusion:These findings suggest that Snail, MHY9, GLUT9, FUT6,HAAO are up-regulated in kidney biopsy of patients with DN and may be involved in the pathogenesis of DN.