The Influence And Mechanism Of FOXQ1 On The Angiogenesis And Proliferation In Colorectal Cancer

Objective : To explore the effect of FOXQ1 gene silencing on angiogenesis and proliferation ability of colon cancer cells and its role in Shh pathway.METHODS: Lentivirus expressing different FOXQ1-shRNA or negative sh RNA was used to infect the SW480 cells. The best silencing condition was screened and used in the following experiments. The SW480 cells were divided into interfered group(FOXQ1-shRNA) and control group(NC-shRNA). The MTT assay was used to observe the growth curve, doubling time and the cell activity. Tube formation assay was performed to detect the ability of angiogenesis. Meanwhile, the expression of VEGF-A, MMP2, cyclinD1 at mRNA and protein levels was determined by RT-qPCR and Western blotting. After induction of the cells byrecombinant Shh proteins, the changes of angiogenesis and proliferation ability in each group were detected. At the same time, the transformation of related gene was examined.RESULTS: Compared with control group, the angiogenic ability in interfered group was weaken, and no obvious difference of proliferation abilitywas observed. The expression of VEGF-A and MMP2 was declined significantly, and the expression of cyclinD1 was not obviously changed. Recombinant Shh proteins improved the expression of FOXQ1 gene. Compared with NC-shRNA group, after induction, the angiogenic ability of FOXQ1-shRNA group was weaken, and the proliferation ability was not obviously changed.CONCLUSION: FOXQ1 gene mediate the angiogenic ability of SW480 cells,but has nothing to do with proliferation. Meanwhile,it maybe regulate by shh pathway.