Effect Of Repetitive Transcranial Magnetic Stimulation On Motor Function In Rast With Kernicterus

PART ONE: BRAIN CELL INJURIES AND BEHAVIORAL CHANGES IN RATS WITH KERNICTERUSObiective: To evaluate the brain cell injuries and behavioral changes of newborn rats with kernicterus.Methods: Twenty-five five-day-old SD rats were divided into normal saline control group(n=11) and kernicterus model group(n=14). Model group was built by injecting bilirubin solution(10ug/g) into the cisterna magna, while control group was injected with equal volume of normal saline(1u L/g). After operation, the neurobehavioral changes of two groups were observed and the bodyweight was recorded; First day after modeling,apoptosis of striatal nerve cells of basal ganglia was checked by TUNEL in model group(n=3). The remaining rats were assessed by gait analysis and beam-walking test after 19 days of age, and Morris water maze test was performed in 30 days of age( n = 11 in each group).Results: 1)After injected with bilirubin, the model group showed apparently abnormal neurobehavioral changes, such as clenched fists,opisthotonos and the bodyweight of model group were lower than the control group on the 3 consecutive days after modeling(P<0.01); 2)The TUNEL: in model group, apoptosis was found in the striatal nerve cells of basal ganglia; 3)The gait analysis: step length in model group rats were shorter than that in controls(P<0.01), while the difference value of step length was greater than control group(P<0.01). There was no statistical difference of step width between the two groups(P>0.05); 4)The beam-walking text: the score of the model group was lower than control group(P<0.01); 5)The Morris water maze test: the escape latency in model group was longer than that in control group(P<0.05), the times of passing through the safe platform in model group were reduced than that in control group(P<0.01).Conclusion: The newborn rats model of kernicterus builted by injecting bilirubin solution into the cisterna magna showed deficits in multiple motor function and learning and memory disorder, which were assessed by gait analysis, beam-walk test and Morris water maze test,respectively.PART TWO: EFFECT OF REPETITIVE TRANSCRANIAL MAGNETIC STIMULATION ON MOTOR FUNCTION IN RAST WITH KERNICTERUSObiective: To investigate whether repetitive transcranial magnetic stimulation(r TMS) can improve the motor function of rats with kernicterus.Methods: Twenty-nine five-day-old SD rats were divided into three groups: normal saline control group(n=7)、kernicterus model group(n=11)and r TMS group(n=11). Model group and r TMS group was injected with bilirubin solution(10ug/g) in the cisterna magna, while control group was injected with equal volume of normal saline(1u L/g). At 19 days of age,Gait analysis was performed on the three groups. The first day after gait analysis( 20 days of age), r TMS group received r TMS treatment daily for7 days. 2000 stimulition from 16 trains in a day. Each train was applied at25 Hz for 5s with 13 s intervals between trains; while Model group received sham- stimulition; No treatment was performed in control group. After 7days of treatment, gait analysis was performed on the 3 groups again.Results: At 19 days of age, left and right step length in model group and r TMS group were shorter than those in control group( P<0.05), while the difference value of step length in model group and r TMS group were greater than those in control group(P<0.05). There was no statistical difference in gait parameters between model group and r TMS group(P>0.05). After 7-day treatment(26 days of age), left and right step length in r TMS group were greater than those in Model group(P<0.05), but shorter than control group(P<0.05); difference value of step length in r TMS group was shorter than that in model group(P<0.05), but greater than control group(P<0.05).Conclusion: r TMS can improve motor function of rats with kernicterus.