Effects Of Bencycloquidium Bromide On The Expression Of MUC5AC Induced By Lipopolysaccharide In Cultured Human Nasal Epithelial Cells

Aim:To investigate the effect of bencycloquidium bromide(BCQB) on mucus MUC5 AC expression induced by lipopolysaccharide in cultured human nasal epithelial cells(HNECs).Methods:Primary culture of human nasal epithelial cells(HNECs) were randomly devided into control group( C,with no treatment), LPS group( LPS,with LPS 1mg·L-1 added in), BCQB low dose group( BCQBL,with LPS 1mg·L-1 and BCQB 10-8 mol·L-1 added in), BCQB middle dose group( BCQBM,with LPS 1mg·L-1 and BCQB 10-7 mol·L-1 added in), BCQB high dose group( BCQBH,with LPS 1mg·L-1 and BCQB 10-6 mol·L-1 added in) and ipratropium bromide group(IB,with LPS 1mg·L-1 and IB 10-6 mol·L-1 added in). After incubation at 37 ℃ with 5% CO2 for 24 h, the expression of MUC5 AC mRNA was detected with Real-time PCR and the expression of MUC5 AC protein in HNECs was detected with Western Blot, while the expression of MUC5 AC protein in supernatant was detected with ELISA in each group.Results:As compared with control group,the expression of MUC5 AC mRNA and protein increased significantly in LPS group(each P <0.01).As compared with LPS group,the expression of MUC5 AC mRNA and protein decreased significantly in each group of BCQB(P <0.01,P <0.05),and there was no statistical difference between BCQB high dose group and control group(each P >0.05).Conclusion:Bencycloquidium bromide can suppress MUC5 AC expression induced by LPS in cultured human nasal epithelial cells,indicating that BCQB may be a new drug for nasal mucous hypersecretion diseases.