Effect Of TXNIP Mediated NLRP3 Inflammasome Activation In The Pathogenesis Of Pe

Objective: To explore the role of TXNIP mediated NLRP3 inflammasome activation in the pathogenesis of PE.Methods: 15 cases of early-onset preeclampsia and 15 cases of normal maternal placental tissue were collected in the First Affiliated Hospital of Chongqing Medical University from December 2013 to June 2014.Western blot was used to detect the expression of TXNIP and NLRP3 in placenta.HTR8/SVneo cells were used to establish the model of PE. After treatment,expression level of TXNIP and NLRP3 were detected by Western blot,caspase 1 activity were measured by caspase-1 active detection kit,interactions between TXNIP and NLRP3 was tested by Co-IP, invasion and proliferation were detected by real-time cell analyzer instrument.Results: The expression of TXNIP and NLRP3 in placenta in PE group was obviously higher than that of normal group(P=0.003,P=0.002).In the cell model, H/R could increase the expression of TXNIP and NLRP3obviously( P=0.000, P=0.001), and interaction between TXNIP andNLRP3 improved remarkably. After inhibition TXNIP, expression of NLRP3 significantly reduced(P=0.000), and ASC level also declined obviously(P=0.001), and partially reversed the ability of invasion and proliferation which decreased in the condition of H/R(P=0.001,P=0.001).Conclusion: TXNIP mediated NLRP3 inflammatory activation plays an important role in the pathogenesis of PE.