Effects Of Perfluorooctyl-bromide Nanoparticles On HIF-1α INR The Early Brain Injury After Subarachnoid Hemorrhage

Objective: The aim of the present study is to investigate the effects of perfluorooctyl-bromide(PFOB) nanoparticles on hypoxia-inducible factor 1 alpha(HIF-1α) protein in the early brain injury(EBI) after subarachnoid hemorrhage(SAH).Methods: According to the principle of random 100 SD rats(n=20) is divided into: Sham, the SAH group, control group, 5mg/kgPFOB group and 10mg/kgPFOB group. A rat of model SAH was created by endovascular perforation and PFOB treatment(5mg/kg or 10mg/kg injected into the caudal vein) was initiated at 1h after SAH. All rats were subsequently sacrificed 24 h after surgery, to evaluate neurobehavioral functional deficit and detect brain water content and the blood-brain barrier(BBB) permeability. Using TUNEL staining to detect neural apoptosis, immunohistochemistry and western blot to detect HIF-1α protein expression, RT-PCR assay of HIF-1α mRNA changes.Results: Treatment with PFOB significantly alleviated EBI(including neurological dysfunction, brain edema, blood-brain barrier disruption and neural cell apoptosis). In addition, it also suppressed the expression of HIF-1α in the rat hippocampus. The effects of 10g/kg PFOB were found to be more obvious than those of 5g/kg PFOB(p < 0.05).Conclusion: Our work demonstrated that PFOB could alleviate EBI after SAH, potentially through downregulation of the expression of HIF-1α, which led to reduced cell apoptosis, BBB disruption and brain edema.