The Effect Of MiR-95 On Invasion And Migration Of Colon Cancer Cell Line SW620 And Its Mechanism

Objective: Acorrding to down-regulate the expression of small molecule noncoding RNA(miRNA-95),to explore the invasion and migration ability of colon cancer SW620 cell line and its mechanism.Methods: The SW620 cells were infected by miR-95 interference lentivirus.The expression of mi R-95 mRNA was confirmed by quantitative real-time polymerase chain reaction.Transwell chambers, scrath test were conducted to detect the ability of cell invasion,migration among each group;and MTT method was used to detect the intercellular adhesion of homogeneous and heterogeneous cells;Western blot was used to detect the expression of EMP1,VEGFC and MMP2 proteins.Results: After the infection of SW620 with miR-95 interference lentivirus for 72 h,significangt fluorescence expression was observed.Compared with empty virus group and ccontrol group,the expression of miR-95 was decreased in transfection group(P<0.05);The invasion and migration ability weakened(P < 0.05);the intercellular adhesion ability of homogeneous cells increased(P<0.05);the intercellular adhesion ability of heterogenous cells decreased(P<0.05).The expression of EMP1 protein in transfection group was obviously higher,VEGFC,MMP2 protein significangtly lower(P<0.05).Conclusion: miR-95 may promote the invasion and migration ability of SW620 cells,its may be achieved by EMP1,VEGFC,MMP2.