Resveratrol Protects Hepatocytes Against Hypoxia Reoxygenation Injury And Its Relationship With The TLR4/NF-κB Pathway

Objective: To explore the protective effect of resveratrol(RES)on hepatocytes induced by hypoxia/reoxygenation(H/R) and the molecular mechanism.Methods: Before the model was established, the BRL-3A cells were treatment with RES or HTA125(the inhibitor of TLR4). survival rate or apoptosis were detected. Alanine transaminase(ALT) and Interleukin-1 beta(IL-1β) in the culture was determined. The mRNA and protein expression level of TLR4 and NF-κB in cells was determined by quantitative real-time PCR or Western blot.Results: The H/R stimulation decreased cell survival rate and enhanced the apoptosis. The levels of ALT and IL-1β were enhanced significantly, and the expression level of TLR4 and NF-κB were markedly increased. After pretreatment with RES or HTA125, the cell survival rate increased while the apoptosis rate decreased, ALT and IL-1β were reduced significantly and the expression level of TLR4 and NF-κBp65 were decreased. Moreover, RES inhibited thetranslocation of NF-κB p65 after the stimulation of H/R in BRL-3A cells.Conclusion: RES can alleviate the hepatocyte injury induced by hypoxia/reoxygenation, which might be mediated in part by TLR4/NF-κB signaling pathway.