Effects Of WNT5B Over-expression On Proliferation And Apoptosis Abilities Of Human Breast Cancer Cell Line MCF-7

Objective : To detect the expression of WNT5 B in normal breast epithelial cells and different breast cancer cell lines, and detect the effects of WNT5 B over-expression on the proliferation and apoptosis of human breast cell line MCF-7 cells.Methods:The expression of WNT5 B mRNA level was detected by RT-PCR in different breast cancer cells. MCF-7 cells were transfected with plasmid pc DNA3.1/WNT5 Band pcDNA3.1) respectively, and the expression of WNT5 B mRNA and protein level were examined in two group by Real-time PCR and Western blotting. Subsequently, the changes of cell proliferation and cell apoptosis were detected by CCK-8 assay and Flow Cytometry respectively.Results:The expression of WNT5 B in breast cancer lines were lower than in MCF10 A cells. The WNT5 B expression in MCF-7 cells in experimental group was significantly higher than that in vector group(P<0.05). However,the abilities of cell proliferation in experimental group decreased significantly as compared with those in vector group(P < 0. 05).The number of cells in S-phase obviously increased while the percentage of cells in G1-phase and G2/M-phase decreased compared with vector group.The number of apoptosis cells in WNT5 B group was significantly increased than that in vector group.Conclusion : The expression of WNT5 B was decreased in breast cancer cells. WNT5 B overexpression significantly inhibited the proliferation and promoted cell apoptosis of breast cancer cell line MCF7.