Expression Of MYH9 In Hepatocellular Carcinoma And Effects Of MYH9 On The Cell Proliferation And Apoptosis In SMMC-7721

Objective: To analyze the expression of nonmuscle myosin heavy chain9(MYH9) in the human hepatocellular carcinoma(HCC) and investigate the effects of RNA interference targeted silence MYH9 gene on the proliferation and apoptosis ability of HCC cell line SMMC-7721.Methods: Immunohistochemistry and western blotting were used to analyze the expression of MYH9 protein in HCC tissues and its cancer-adjacent tissues, and the relationship between MYH9 and clinical pathological parameters was studied. Western blotting was also used to analyze the expression of MYH9 protein in HCC cell lines SMMC-7721, Hep G2 and normal liver cell LO2. RNA interference targeted silence MYH9 gene was transfected into SMMC-7721 cells, The proliferation and apoptosis of the transfected cells were assessed by CCK-8 assay and flow cytometry.Results: The results of immunohistochemistry and western blotting demonstrated that the expression of MYH9 in HCC tissues was found to be significantly higher than that of cancer-adjacent tissues, and the expression of MYH9 protein was correlated with tumor size, portal vein invas ion, TMN stages and Edmondson grade(P<0.05). The relative amount of MYH9 protein in SMMC-7721 and Hep G2 was significantly higher than that in LO2. It was showed that downregulation of MYH9 inhibited cell proliferation(P<0.001) and induced the apoptosis(P<0.05).Conclusion: The expression of MYH9 protein in HCC tissues was significantly higher than that in cancer-adjacent tissues, and RNAi of MYH9 in the SMMC-7721 cells may inhibit cells proliferation and induce apoptosis.