The Expression And Significance Of MyD88 In Theartery Tissues With Buerger’s Disease

Objective: To observe the expression and distribution of myeloid differentiation primary-response gene 88(MyD88)in the artery between Buerger’s disease and control group. Discover the correlation of MyD88 and Buerger’s disease. Study the mechanism of MyD88 in the pathogenetic process of Buerger’s disease.Methods: 9 cases patients with Buerger’s disease undergoing the artery resection from July2013 to July2015were gathered as Buerger’s disease group, and12 cases patients with malignant tumor or injury undergoing the artery resection were recruited as controls. The mRNA and protein levels of MyD88 were measured using the methods of RT-PCR and Western blot, respectively. In addition, the characteristic of MyD88’s distribution was observed using the methods of immunohistochemistry and immunofluorescence.Results: According to the results of RT-PCR and Western Blot, the level of MyD88 expressed in the artery tissues of Buerger’s disease was obviously higher than in the control tissues(P<0.01).The results of immunohistochemistry and immunofluorescence further showed MyD88 protein was mainly expressed in the smooth muscle cells and endothelial cells in the artery tissues with Buerger’s disease.Conclusion: The mRNA and protein level of MyD88 were strongly up-regulated in the arteries with Buerger’s disease as compared with controls. the MyD88 protein primarily distributed in the arterial smooth muscle cells and the endothelial cells in Buerger’s disease. These results indicate that MyD88 is probably related to Buerger’s disease and the signaling pathway of TLRs-MyD88 maybe play a key role in the development of Buerger’s disease.