| ObjectiveInterleukin(IL)-22 is a member of the IL-10 superfamily. Recently study has reported that IL-22 is associated with behcet’s disease which classified as a type of vasculitis. However, further studies are required to determine which effects does IL-22 take on human umbilical vein endothelial cells(HUVECs). The purpose of this study was to investigate the effects of IL-22 on cytokine production, proliferation, and barrier function of HUVECs.MethodsWe chose inflammation induced by LPS on HUVECS as a model of vasculitis cells.The expression of IL-22R1 m RNA in HUVECS was detected by semiquantitative reverse transcription-polymerase chain reaction(RT-PCR). Real-time quantitative PCR technique and Western blot were used to detect the expression of Cytokine. The proliferation ability of HUVECS was evaluated using a cell counting kit assay. The endothelial barrier permeability was assessed by measuring permeability toFITC-labeled dextran. The distribution of occludin was detected by immunofluorescence.Results:IL-22R1 m RNA expression was noted in HUVECS. IL-22 also significantly enhanced the production of CCL2 and CCL20 by HUVECS,but had no effect on expression of IL-6 and IL-8. After treatment with IL-22, the HUVECS showed increased proliferation activity, and IL-22 could effectively suppress LPS-induced proliferation inhibition. Besides,IL-22 could improved barrier function and decreased LPS-induced increased cellular permeability and inhibited the LPS-induced destruction of occludin in HUVECs.ConclusionsIL-22 could enhanced the production of CCL2 and CCL20 which known as chemotactic cytokines by HUVECs. While IL-22 had no effect on expression of IL-6 and IL-8 which known as inflammatory factors.Besides, IL-22 could not only significantly enhance the proliferation ability,but also improved barrier function of HUVECs by increasing the protein expression of occludin. These results suggest that IL-22 may play a protective role in vasculitis. |
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