Circular Genetic Information And Biologic Characteristics Of Mycobacteriophage Guo1

[Objective] To investigate the circular genetic information and biologic characteristics of mycobacteriophage Guo1[Methods] Measured the optimal multiplicity of infection and one-step growth curve and observed the sensibility to ultraviolet, chloroform, alcohol, temperature, pH. Sequenced the complete genome of Guo1 with Shot-gun library and config package strategy. Performed DNAStar, tRNAscan-SE, Promoter predictions, TRF, Glimmer, BLAST software for the general genome characteristics, tRNA, promoter regions, tandem repeat sequences and putative function of predicted coding sequences, respectively. Utilized BLAST software to search for mycobacteriophages similar as Guo1. Constructed phylogenetic tree to determine which cluster Guo1 should be grouped into.[Results] Guo1 is resistant to ultraviolet but sensitive to alcohol, chloroform and hot temperature. The optimal temperature is 37 °C while the optimal p H is 7.0. The optimal MOI of Guo1 is about 0.01. One step growth of it showed that both latent period and burst period are 120 min, and the average burst size is about 47. Guo1 contained a double strand circular DNA with a full length of 40 086 nt, which belonged to the cluster G mycobacteriophage and which contained 59 putative genes, 7 promoter regions and 3 tandem repeat sequences.[Conclusion] Guo1 is one of cluster G mycobacteriophage with double strand circular DNA found for the first time. We sequenced the complete genome of Guo1 and identified the circular genetic information and biologic characteristics, which indicated Mycobacteriophage Guo1 has the potential to resuscitate and kill mycobacterium tuberculosis.