Effcts Of Different Lighting On Reproductive System In Depressive Rats

ObjectiveTo investigate the effects of different lighting on reproductive system in depressive rats. The first partTo investigate the effects of different lighting on reproductive system in depressive male rats. Methods:Healthy adults male rats(n=7) were randomly chosen as control group, and the depressive adults male rats(n=35) were randomly divided into 5 groups(7 rats each):depressive model group, sulfur lamp group, heat radiation lamp group, fluorescent lamp group and LED lamp group. The body weight and general behaves of rats were recorded during the experiment. After 45 d of continuous illumination, serum was collected and reproductive organs were removed from rats, then the organ index of testicle, epididymis, seminal vesicle and kedney was calculated, the concentrations of testosterone(T),estiadrol(E2), prolactin(PRL) and progesterone(PROG)in the serum were detected by ELISA, the histopathological lesion of testicle was observed under microscope with HE staining,the expression of 3β-HSD and melatonin receptors(Mel1a) of testicle were detected by Western blotting. Results:1. The body weight of depressive rats increased significantly during the light therapy.2. The organ Index of testicle,epididymis and seminal vesicle, and concentration of T、E2、PRL and PROG in the depressive rats were obviously lower than control group(P<0.05), and seminiferous tubules were atrophied in the testicle of the depressive rats.3. Histopathological damage of testicle were obvious improved, concentrations of T, E2, PRL and PROG and the expression of 3β-HSD and Mel1 a were significantly increased in the depressive male rats treated with the sulfur lamp lighting(P<0.05).4. There were no obvious change in the organ index, histopathological lesion of testicle, and concentration of T、E2、PRL and PROG in the serum when the depressive rats were treated with lighting of the heat radiation lamp, or the fluorescent lamp, or the LED lamp. The second partTo investigate the effects of different lighting on reproductive system in depressive female rats. Methods:Healthy adults female rats(n=7) were randomly chosen as control group, and the depressive adults male rats in SPF grade(n=35) were randomly divided into 5 groups(7 rats each):depressive model group, sulfur lamp group, heat radiation lamp group, fluorescent lamp group and LED lamp group. Record body weight of rats during the experiment.After 45 d of continuous illumination,the estrous cycle was observed by the vaginal exfoliated cells, the organ index of ovary and uterus was calculated, the concentrations of E2, PRL, PROG and T in the serum were detected by ELISA, the histopathological lesion of ovary was observed under microscope with HE staining. Results:1. The gaining of body weight of the experimental rats were less during stressing(P<0.01). The reproductive functions of depressive rats were serious damaged by stress stimuli, which include serious disorder of estrous cycle, the lower concentration of E2, PRL, PROG, T in the serum, histopathological lesion of the ovary.2. After lighting therapy the body weight of rats increased significantly.3.The estrous cycle and histopathological damage of ovary were obvious improved, and concentrations of E2, PRL, PROG and T were significantly increased after the sulfur lamp lighting in the depressive female rats compared with depressive model group(P<0.05).4. There were no obvious improvements in the organ index, histopathological lesion of the ovary, and concentration of sex hormones in the serum when the depressive rats were treated with lighting of the heat radiation lamp, or the fluorescent lamp, or the LED lamp. Conclusion:The reproductive functions of depressive rats can been improved by sulfur lamp lighting.