Influence Of SOCS1、SOCS3 On The Imbalance Of Th1/Th2 In Children With Henoch-Sch(?)nlein Purpura

Objective To investigate the roles of suppressor of cytokine signaling(SOCS) 1, SOCS3 in the imbalance of Th1/Th2 in children with Henoch- Sch(?)nlein purpura(HSP).Methods 32 children with acute phase HSP and 28 age-matched healthy children were enrolled in this study. The level of p STAT3 protein in peripheral blood mononuclear cells(PBMCs) was detected by Western blot. The expression levels of SOCS1 m RNA 、SOCS3m RNA in PBMCs were analyzed by real-time PCR. The levels of IL-4、IFN-γand IL-6 in plasma were measured by ELISA.Results 1. The levels of IFN- γ in HSP group were significantly lower than those in control group(p<0.01),the levels of of IL-4 were significantly higher than those in control group(p<0.01),and the ratio of IFN-γ/IL-4 in HSP group were significantly lower than those in control group(p<0.01). 2. The levels of IL-6 and phosphorylated STAT3(p STAT3)protein expression in PBMCs of HSP group were markedly higher than those of control group(p<0.01). 3. The expression of SOCS1 m RNA and SOCS3 m RNA in children with HSP were significantly higher than those in healthy control group(p<0.01). 4. The p STAT3 protein in HSP group positively correlated with SOCS1 m RNA(P < 0.01), and showed a trend of positive correlation with SOCS3 m RNA, though it was not statistically significant(P>0.05).Conclusions 1. There exists an imbalance of Th1/Th2 in acute phase of HSP. 2.IL-6/STAT3 pathway is activated in acute phase of HSP, and SOCS1 and SOCS3 are abnormally increased in children with HSP, which may be an important factor in the formation of the imbalance of Th1/Th2 in children with HSP.