The Effect Of Matrine On HepG2 Liver Cancer Cell Proliferation And Migration By ERK Signaling Pathway

Objective: To observe the inhibitory effect and mechanism of matrine on liver cancer. Method: Chose Hep G2 liver cancer cell lines and select the better concentration gradients of matrine by MTT. Observe the influence of matrine on cell morphology of Hep G2.Then we divide experiment into blank control group which isn’t dealed with matrine, experimental group which is dealed with 1m L matrine concentration by 1, 2 and 4 mg/m L respectively and positive control group which is dealed with 1m L PD98059, an ERK signaling pathway blockers, by 20 umol/L. MTT detects cell proliferation, Transwell detects cell migration, reverse transcription polymerase chain reaction detects the expresstion of ERK m RNA and Western-Blot detects the protein expression changing of ERK and p-ERK.Result:1. The cell morphological changes: With the increase of matrineconcentration, the density and amount of Hep G2 cell decreased. Cell shape is irregular. On the surface of nutrient solution some necrotic cellular debris can be seen.2. Detected by MTT, according to the results of matrine inhibiting Hep G2 cell proliferation has the time and the concentration dependence. During 24 h, the sensitive concentration of matrine is 1 mg/m L, 2 mg/m L, 4 mg/m L.3. The ability of cell migration changes: Comparing experiment group with the blank control group, the average cell migration of 1 mg/m L、2 mg/m L、4 mg/m L matrine concentration decrease strikingly(P <0.01). Comparing experiment group with the positive control group, while there is no difference between 4 mg/m L group and the positive control group(P >0.05).4. ERK m RNA expression: ERK m RNA expression was significantly decreased after dealed with matrine. Compared experiment group with the blank control group and positive control group, the difference of 4 mg/m L、 2 mg/m L concentration is statistically significant(P <0.05).5. The protein expression of ERK and p-ERK: Comparing experiment group with the blank control group, the protein expression of p-ERK in 2 mg/m L, 4 mg/m L concentration has statistical significance(P <0.01) and ERK protein expression in 2 mg/m L, 4 mg/m L concentration had statistical difference(P <0.05). Comparing experiment group with the positive control group, 1 mg/m L, 2 mg/m L group has statistical difference(p< 0.05), and 1 mg/m L has a significant difference(P<0.01).Conclusion:1. Matrine inhibits Hep G2 liver cancer cell proliferation and migration, its specific mechanism may cut ERK signal transduction pathway through the expression of key protein ERK and p-ERK.2. The effect of matrine on Hep G2 liver cancer cell inhibition has time and concentration dependence.