The Influence In Peritonitis Complicated With Lung Damage Of Mice By Removing Neutrophils

Objective:1,To study the severity of acute lung injury by establishing peritonitis model of the normal mice.2,To injecting antibody drugs to vein of mice’s tail to remove neutrophils.3,After removing the neutrophils of mice, to study the acute lung injury of mice by establishing peritonitis models,and establishing the set up blank control group at the same time. Through observing and comparing the extent of acute lung injury of mice after giving them the different treatment to study the removal of neutrophils in the influence of secondary bacterial peritonitis complicated with acute lung injury in mice.Methods:1, The 80 clean and healthy mice were divided into two groups of 40, body weight at 20-23g,as removal of mouse neutrophils in mice group(N), pseudo removal of mouse neutrophils group (T).2,The mice of group N were injected the single cell cloning antibody reagents, Ly6G-1A8, into the tail vein.Then blood the mice and test the blood of them before or after the injection. At the same time,record the result of mouse blood and the neutrophil count.3, To Inject different drugs into the abdominal cavity of the four groups’mice 24 hours after tail vein injection.Besides,they should have been food ban 12 hours,but they can drink water.4,The mice of group ’N’and group’T’ were then divided into two groups-peritonitis group and pseudo-peritonitis group, a total of four groups of 20 mice each, labeled neutrophils removed peritonitis group (NP), the removal of neutrophils pseudo peritonitis group (NS), does not remove the neutrophils peritonitis (TP), does not remove the neutrophils pseudo-peritonitis group (TS).5, Groups ’NP’ and’TP’should be established coli peritonitis models.Instead of E.coli,we use saline to inject into mouse peritoneall of group ’NS’ and group ’TS’. Mcfarland standard turbidimetric tube with the count made 3xl08cfu/ml concentration of Escherichia coli.6,By observing the deaths and histopathology of four groups’ mice to evaluate the extent of the all mouse lung injury.Use ascites, liver, spleen tissue of all mice to culture bacterial and identify during sterile environment.7, Use the liver, spleen tissue for pathological to observe peritonitis.8, Take ascites liver, spleen tissue of four groups’mice under sterile conditions to make bacterial culture and identification.Results:1, After maked peritonitis models of mice, the groups of NP and TP had mice died,but the NS and blank group mice(TS) had no mice died. Rrsult is that60%,75%,0% and 0%.Compared the two peritonitis mice,the mortality rate of group’NP’ is higher than the group ’TP’. The two groups mice with peritonitis were dissected.Then they could be see too much pleural effusion.They have higher Lung wet-dry ratio(2.74±0.43vs3.92± 0.88)and low Oxygen saturation(443.22 ± 28.20 vs 250.58 ± 23.01)(p<0.01).They all have ALI,and the group of’TP’ were more serious(5.4±0.8 vs7.8± 1.3)(p<0.01).Other two groups had no death and no lung injury after being maked dummy peritonitis model, (NS and TS). The two groups’mice were dissected. We can see they have little pleural effusion, lung wet-dry ratio is low, and Oxygen saturation is normal.Besides, their lung, liver and spleen biopsy had no abnormal. Use The Intraperitoneal and Liver and spleen tissue of all mice to culture out bacteria and identify.The two Peritonitis groups could be cultured bacteria,that are E. coli,but other groups had no bacterial growth.Conclusion:1,We can use single cell clone antibody, Ly6G-lA8,to move neutrophil of mice.2,Moving neutrophils of mice can reduce mortality of E. coli peritonitis models. The mechanism may be associated with decreased lung injury in peritonitis mice...