Research On Gold Nanoparticles Improving The Drug-resistance Of Colon Carcinoma Cells And Its Mechanism

Objective To observe gold nanoparticles affect oxaliplatin against to drug-resistant colon carcinoma cells and the related mechanism research preliminarily.Methods GNPs was synthetised by chemical method and identified by UV-Vis spectrophotometer and transmission electron microscopy(TEM). The first step of experiment is to test the sensitivity to L-OHP 、the expression of gene MDR、MRP1 and P-glycoprotein by MTT、PCR and Western blot respectively between COLO 205/L-OHP and COLO 205/S; The second step is to deal with COLO 205/L-OHP using GNPs combined with L-OHP, detecting its proliferation inhibitory rate and apoptosis rate with MTT and flow cytometry. The third step is to measure the effect of different concentration GNPs against COLO 205/L-OHP, observing the changes of b-FGF,bcl-2 in cells, using ELISA and Western blot respectively.Results The maximum absorption peak of GNP is 520 nm, the diameter is about 10 nm; The sensitivity of COLO 205/L-OHP is lower than COLO 205/S(P<0.05)in L-OHP with the concentration 1.5 μg/mL L-OHP; Moreover, the expression of gene MDR、MRP1 and P-gp in COLO 205/L-OHP is higher than COLO 205/S(P<0.05). Compare with L-OHP alone treated the proliferation inhibitory rate and apoptosis rate of COLO 205/L-OHP were higher by GNPs combined with L-OHP treated, and GNPs have no effect for the two index(P>0.05). The concentration of b-FGF in cell culture fluid is higher in COLO 205/L-OHP compared with COLO 205/S(P<0.05), further the b-FGF decreased after treated with different concentration of GNPs in a dose-dependent manner. The similar situation also can be seen as the expression of b-FGF and Bcl-2 in the two cell lines.Conclusions Gold nanoparticles can reduce the content of b-FGF, decrease the expression of Bcl-2 in COLO 205/L-OHP to add chemotherapy medicine toxicity,can improving the situation of drug-resistance.