| Objective: To investigate the effects of dexmedetomidine preconditioning on mitochondrial function of myocardial ischemia reperfusion injury(MIRI), and to explore the protective effect of dexmedetomidine preconditioning on myocardium with ischemia and reperfusion injure.Methods: 30 healthy male Sprague Dawley(SD) rats, aged 8 weeks, were randomly divided into three groups: sham operation group(group A), myocardial ischemia-reperfusion group(group B), dexmedetomidine preconditioning group(group C). The model of myocardial ischemia-reperfusion injury was performed by ligation of left anterior descending coronary artery. Cardiac functions were detected by echocardiography. The expression of the m RNA levels assosiated with mitochondrial function was performed by real-time PCR. Ultrastructure of myocardial mitochondria was observed under electronic microscope.Results: Compared with group A, the cardiac function of group B and group C were markedly decreased; the m RNA levels of genes related to mitochondrial function were significantly lower in group B and group C than in group A(P <0. 05).Compared to group B, the cardiac function of group C was enhanced; the m RNA levels of genes related to mitochondrial function were higher in group C than in group B(P <0.05). We also found that impaired mitochondrial ultrastructure was observed in group B. Compared with group B, impaired mitochondrial structure was significantly improved in group C. Conclusion: Dexmedetomidine preconditionning could improve mitochondrial function to alleviate myocardial ischemia-reperfusion injury.Conclusion: Dexmedetomidine preconditionning could improve mitochondrial function to alleviate myocardial ischemia-reperfusion injury. |
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