Circulating MicroRNA-499 And MicroRNA-92a In Patients With Acute Coronary Syndrome

Objective:To explore the regulation of circulating micro RNA-499 and micro RAN-92 a in patients with acute coronary syndrome(ACS). Then discuss the relationship between the concentration of miR-499/miR-92 a and the Gensini scores.Methods:A total of 60 patients undergoing coronary angiography in the catheterization laboratory of the first hospital of Shanxi Medical University, from January 2015 to August 2015 were enrolled. Patients were classified into three groups according to the results of ECG, evidence of CAD at angiography and cardiac troponin I(cTnI) levels.EDTA-plasma were collected and stored at-80℃. The concentration of miR-499 and miR-92 a were detected using SYBR qRT-PCR method by relative quantitative analysis.Results:The baseline condition was similar between three groups(P>0.05). Circulating levels of the miR-499 were significantly elevated in the troponin-positive acute coronary syndrome patients(AMI patients) compared with patients with unstable angina and non-CAD. Circulating levels of the miR-92 a were significantly decreased in the troponin-positive acute coronary syndrome patients(AMI patients) compared withpatients with unstable angina and non-CAD.In the group of AMI patients, miR-499 and miR-92 a concentration were correlated with cTnI、NT-proBNP、hs-CRP,but not with the Gensini scores.Conclusion:1.micro RNA-499 is released from the cardiomyocytes into the coronary circulation on myocardial injury. As a potential biomarker for acute myocardial infarction, plasma miRNA-499 may be useful in predicting AMI.2. micro RNA-92 a may serve as a new class of blood-based biomarkers of AMI. Moreover, miR-92 a concentration can not reflect the severity of coronary stenosis in patients with acute coronary syndrome.