The Effects Of PDGF-BB And β-PDGFR Expression By Yin Chen Gao Decoction On Liver Fibrosis Model Rats

Objective: Observation of CCl4 induced rat experimental liver fibrosis in the process of the formation and development, the change of serum PDGF-BB, β-PDGFR, explore the artemisia capillaris decoction on serum PDGF-BB, the influence of theβ-PDGFR expression.Methods: 45 clean level only SD male rats were randomly divided into control group and model group, model group using 40% CCl4 was produced by intraperitoneal injection of 0.1 ml/100 g the liver fibrosis model. 1 time every Monday and Thursday, 3 weeks in a row, made during the mold by intraperitoneal injection of the control group given the same amount of olive oil.In building the third weekend model group were randomly divided into model group, control group and artemisia capillaris soup of artemisia capillaris soup group to continue building at the same time give the artemisia capillaris soup 10 ml/kg(3.886 g/kg) of crude drug intervention treatment, 1 x per day, 2 weeks in a row, abdominal continue injecting CCl4 model control group during the period of intervention at the same time give the same amount of saline stomach, abdominal continue to normal control group injected with olive oil at the same time give the stomach the same amount of saline solution.Selection during the experiment, 1, 2, 3, 4, 5, 5 point in time during the weekend each rat abdominal aortic blood, using ELISA method to detect each group of rats serum cytokines PDGF-BB, β-PDGFR and variation of the liver function of AST, ALT, each team will gather the rat liver tissue by using HE staining and Sirius staining to observe the liver tissue pathology change.Results: ①Compared with normal group, model group significant liver tissue damage is liver cirrhosis and liver fibrosis, and further development.PDGF-BB, β-PDGFR content increased, to reach a peak at 4 weeks(P < 0.01);Liver function index AST and ALT levels increased, reached a peak to 3 weeks(P < 0.01);② compared with the building modules, artemisia capillaris soup group of liver tissue injury and the degree of liver fibrosis significantly improve, PDGF-BB, β-PDGFR, and the expression level of AST, ALT decreased significantly, basic maintenance level in building 1 week group(P < 0.01, P < 0.05).Conclusion: ①In the formation and development of liver fibrosis in rats induced by CCl4, PDGF-BB, β-PDGFR expression level increased, prompt PDGF- BB, β-PDGFRand CCl4 induced the formation and development of liver fibrosis in rats.② the artemisia capillaris soup can protect liver function and the PDGF-BB, β-PDGFR mediated signal transduction pathways in the cells inside and outside, to reduce the experimental liver fibrosis in rats serum cytokines PDGF-BB, β-PDGFR the expression level of AST, ALT and liver function indicators, to reverse or delay the onset of liver fibrosis in rats induced by CCl4 and development, improve the liver tissue pathology, and liver function.